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Vol 60, No 2 (2022)
Original paper
Submitted: 2021-07-27
Accepted: 2022-05-18
Published online: 2022-05-30
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A chronic moderate methionine administration induced hyperhomocysteinemia associated with cardiovascular disease phenotype in the sand rat Psammomys obesus

Fouzia Zerrouk1, Billel Chaouad1, Adel Ghoul1, Naima Chalour2, Anissa Moulahoum1, Zineb Khiari1, Mohamed el Hadi Cherifi3, Souhila Aouichat4, Karim Houali5, Yasmina Benazzoug1
DOI: 10.5603/FHC.a2022.0013
·
Pubmed: 35603572
·
Folia Histochem Cytobiol 2022;60(2):111-124.
Affiliations
  1. Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene University of Science and Technology (USTHB), Bab Ezzouar, Algiers, Algeria
  2. Laboratory of Physiology of Organisms, Faculty of Biological Sciences, Houari Boumediene University of Science and Technology (USTHB), Bab Ezzouar, Algiers, Algeria
  3. Central Laboratory of Biology, EPH Bologhine Ibn Ziri, Algiers, Algeria
  4. Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, Houari Boumediene University of Science and Technology (USTHB), Bab Ezzouar, Algiers, Algeria
  5. Laboratory of analytic biochemistry research and biotechnology, Faculty of Biological Sciences and Agronomic Sciences, Mouloud Mammeri University, Tizi-Ouzou, Algeria

open access

Vol 60, No 2 (2022)
ORIGINAL PAPERS
Submitted: 2021-07-27
Accepted: 2022-05-18
Published online: 2022-05-30

Abstract

Introduction. Cardiovascular diseases were defined as coronary artery, cerebrovascular, or peripheral arterial disease. Hyperhomocysteinemia (Hhcy) is an independent risk factor of cardiovascular diseases, including atherosclerosis. Our previous studies demonstrated the involvement of Hhcy in cardiovascular remodeling in the sand rat Psammomys obesus. Material and methods. An experimental Hhcy was induced, in the sand rat Psammomys obesus, by a daily intraperitoneal injection of 70 mg/kg of methionine for a total duration of 6 months. The impact of Hhcy on the cellular and matrix structures of the heart, aorta and liver was analyzed using histological techniques. Additionally we treatedprimary cultures of aortic smooth muscle cells (SMCs) with high concentration of methionine to investigate the effects of methionine at the cellular level. Results. A moderate Hhcy induced a significant increase in the extracellular matrix components particularly collagens which accumulated in the interstitial and perivascular spaces in the studied organs indicating a developing fibrosis. A liver steatosis was also observed following methionine treatment. Further analysis of the aorta showed that Hhcy also induced vascular alterations including SMCs reorientation and proliferation associated with aneurysm formation. Conclusions. Our results show for the first time that Hhcy can induce a cardiovascular and liver diseases phenotype in Psammomys obesus, a species previously shown to be a good model for the studies of diabetes and other metabolism-related pathologies.

Abstract

Introduction. Cardiovascular diseases were defined as coronary artery, cerebrovascular, or peripheral arterial disease. Hyperhomocysteinemia (Hhcy) is an independent risk factor of cardiovascular diseases, including atherosclerosis. Our previous studies demonstrated the involvement of Hhcy in cardiovascular remodeling in the sand rat Psammomys obesus. Material and methods. An experimental Hhcy was induced, in the sand rat Psammomys obesus, by a daily intraperitoneal injection of 70 mg/kg of methionine for a total duration of 6 months. The impact of Hhcy on the cellular and matrix structures of the heart, aorta and liver was analyzed using histological techniques. Additionally we treatedprimary cultures of aortic smooth muscle cells (SMCs) with high concentration of methionine to investigate the effects of methionine at the cellular level. Results. A moderate Hhcy induced a significant increase in the extracellular matrix components particularly collagens which accumulated in the interstitial and perivascular spaces in the studied organs indicating a developing fibrosis. A liver steatosis was also observed following methionine treatment. Further analysis of the aorta showed that Hhcy also induced vascular alterations including SMCs reorientation and proliferation associated with aneurysm formation. Conclusions. Our results show for the first time that Hhcy can induce a cardiovascular and liver diseases phenotype in Psammomys obesus, a species previously shown to be a good model for the studies of diabetes and other metabolism-related pathologies.

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Keywords

Cardiovascular diseases; Hyperhomocysteinemia; Liver; Methionine; Psammomys obesus; SMCs

About this article
Title

A chronic moderate methionine administration induced hyperhomocysteinemia associated with cardiovascular disease phenotype in the sand rat Psammomys obesus

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 2 (2022)

Article type

Original paper

Pages

111-124

Published online

2022-05-30

Page views

4691

Article views/downloads

516

DOI

10.5603/FHC.a2022.0013

Pubmed

35603572

Bibliographic record

Folia Histochem Cytobiol 2022;60(2):111-124.

Keywords

Cardiovascular diseases
Hyperhomocysteinemia
Liver
Methionine
Psammomys obesus
SMCs

Authors

Fouzia Zerrouk
Billel Chaouad
Adel Ghoul
Naima Chalour
Anissa Moulahoum
Zineb Khiari
Mohamed el Hadi Cherifi
Souhila Aouichat
Karim Houali
Yasmina Benazzoug

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