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Vol 60, No 1 (2022)
Original paper
Submitted: 2021-09-28
Accepted: 2021-12-27
Published online: 2022-01-17
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EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells

Ying Li12, Jiuxiang Feng13, Yue Bian1, Wei Cheng2, Chong Qiao1456
DOI: 10.5603/FHC.a2022.0001
·
Pubmed: 35038162
·
Folia Histochem Cytobiol 2022;60(1):31-43.
Affiliations
  1. Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China
  2. Department of Obstetrics, Dalian Maternal and Child Health Care Hospital, Dalian, Liaoning, China
  3. Department of Gynecology, Dalian Maternal and Child Health Care Hospital, Dalian, Liaoning, China
  4. Key Laboratory of Maternal-Fetal Medicine of Liaoning Province, Shenyang, Liaoning, China
  5. Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Shenyang, Liaoning, China
  6. Research Center of China Medical University Birth Cohort, Shenyang, Liaoning, China

open access

Vol 60, No 1 (2022)
ORIGINAL PAPERS
Submitted: 2021-09-28
Accepted: 2021-12-27
Published online: 2022-01-17

Abstract

Introduction. Pre-eclampsia is a pregnancy-specific syndrome, which is partly due to abnormal proliferation and invasion of trophoblast cells. EP300 interacting inhibitor of differentiation 1 (EID1) participates in cell proliferation and invasion. This study aims to investigate the roles of EID1 in trophoblast cells and pre-eclampsia. Material and methods. The expression of EID1 in placental tissues from 60 women with pre-eclampsia and 60 health pregnancies was detected by real-time PCR and immunohistochemical staining. EID1 was overexpressed or silenced by transfection of plasmid or siRNA in HTR-8/SVneo trophoblast cells, and then cell proliferation, cell cycle transition, migration, and invasion were determined by CCK-8 assay, flow cytometry, immunofluorescent staining, immunoblotting, and transwell assays. In addition, the activity of Akt/b-catenin signaling was measured by immunofluorescent staining and Western blot. Results. EID1 mRNA level was decreased in placental tissues of pre-eclampsia patients, especially early-onset pre-eclampsia, accompanied by more severe clinical manifestation and a higher rate of fetal growth restriction (FGR). Gain- and loss-of-function experiments demonstrated that EID1 promoted proliferation and cell cycle transition, migration, and invasion in HTR-8/SVneo cells and its knockdown played opposite roles, suggesting that EID1 may be required for normal gestation. Akt/b-catenin signaling was activated after EID1 forced expression and deactivated after its silencing. Conclusions. EID1 promoted proliferation and invasion of cultured trophoblast cells with possible involvement of Akt/b-catenin signaling. These findings may provide novel insights for the diagnosis and treatment of early-onset pre-eclampsia in a clinic.

Abstract

Introduction. Pre-eclampsia is a pregnancy-specific syndrome, which is partly due to abnormal proliferation and invasion of trophoblast cells. EP300 interacting inhibitor of differentiation 1 (EID1) participates in cell proliferation and invasion. This study aims to investigate the roles of EID1 in trophoblast cells and pre-eclampsia. Material and methods. The expression of EID1 in placental tissues from 60 women with pre-eclampsia and 60 health pregnancies was detected by real-time PCR and immunohistochemical staining. EID1 was overexpressed or silenced by transfection of plasmid or siRNA in HTR-8/SVneo trophoblast cells, and then cell proliferation, cell cycle transition, migration, and invasion were determined by CCK-8 assay, flow cytometry, immunofluorescent staining, immunoblotting, and transwell assays. In addition, the activity of Akt/b-catenin signaling was measured by immunofluorescent staining and Western blot. Results. EID1 mRNA level was decreased in placental tissues of pre-eclampsia patients, especially early-onset pre-eclampsia, accompanied by more severe clinical manifestation and a higher rate of fetal growth restriction (FGR). Gain- and loss-of-function experiments demonstrated that EID1 promoted proliferation and cell cycle transition, migration, and invasion in HTR-8/SVneo cells and its knockdown played opposite roles, suggesting that EID1 may be required for normal gestation. Akt/b-catenin signaling was activated after EID1 forced expression and deactivated after its silencing. Conclusions. EID1 promoted proliferation and invasion of cultured trophoblast cells with possible involvement of Akt/b-catenin signaling. These findings may provide novel insights for the diagnosis and treatment of early-onset pre-eclampsia in a clinic.

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Keywords

pre-eclampsia, trophoblast cells, EP300 interacting inhibitor of differentiation 1, invasion, pregnancy

About this article
Title

EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 1 (2022)

Article type

Original paper

Pages

31-43

Published online

2022-01-17

Page views

5850

Article views/downloads

499

DOI

10.5603/FHC.a2022.0001

Pubmed

35038162

Bibliographic record

Folia Histochem Cytobiol 2022;60(1):31-43.

Keywords

pre-eclampsia
trophoblast cells
EP300 interacting inhibitor of differentiation 1
invasion
pregnancy

Authors

Ying Li
Jiuxiang Feng
Yue Bian
Wei Cheng
Chong Qiao

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