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Vol 59, No 1 (2021)
Original paper
Submitted: 2020-01-30
Accepted: 2020-09-18
Published online: 2021-01-27
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Reactivity of astrocytes in hippocampal CA1 area in rats after administration of habanero peppers

Jadwiga Jaworska-Adamu1, Aleksandra Krawczyk1, Karol Rycerz1, Marcin Golynski2
DOI: 10.5603/FHC.a2021.0001
·
Pubmed: 33502751
·
Folia Histochem Cytobiol 2021;59(1):1-7.
Affiliations
  1. Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland
  2. Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Toruń, Poland

open access

Vol 59, No 1 (2021)
ORIGINAL PAPERS
Submitted: 2020-01-30
Accepted: 2020-09-18
Published online: 2021-01-27

Abstract

Introduction. Astrocytes react to microenvironmental changes. Their reactivity is manifested by an increase in glial fibrillary acidic protein (GFAP) and S100b protein levels, hypertrophy and hyperplasia. The aim of the study was to analyse immunoreactive GFAP (GFAP-IR) and S100b (S100b-IR) astrocytes of hippocampal CA1 area in adult rats intragastrically (i.g.) treated with habanero peppers.

Material and methods. Brains from 10 control rats (group C) and 10 rats receiving oil suspension of habanero fruits for 7 days (group I-7) or 28 days (group II-28) were used. Antibodies against GFAP and S100b were used for immunohistochemistry. Morphology and distribution of astrocytes was evaluated under light microscope and their density was quantitatively analysed.

Results. In the CA1 hippocampal area of group II-28 rats, GFAP-IR cells with numerous, branched processes were observed. S100b-IR astrocytes had delicate, single processes in comparison with cells without processes observed in groups I-7 and C. In groups I-7 and II-28, GFAP-IR astrocytes’ density significantly increased in SR — stratum radiatum of hippocampal CA1 area. In group I-7, a density of cells with the expression of S100b was significantly increased in SO — stratum oriens layer. In group II-28, the density of S100b-IR astrocytes was decreased.

Conclusions. Habanero peppers administrated to rats, especially for a longer time, caused reactive changes in the astrocytes in hippocampal CA1 area, and thus these glial cells may protect neurons against excitotoxic damage.

Abstract

Introduction. Astrocytes react to microenvironmental changes. Their reactivity is manifested by an increase in glial fibrillary acidic protein (GFAP) and S100b protein levels, hypertrophy and hyperplasia. The aim of the study was to analyse immunoreactive GFAP (GFAP-IR) and S100b (S100b-IR) astrocytes of hippocampal CA1 area in adult rats intragastrically (i.g.) treated with habanero peppers.

Material and methods. Brains from 10 control rats (group C) and 10 rats receiving oil suspension of habanero fruits for 7 days (group I-7) or 28 days (group II-28) were used. Antibodies against GFAP and S100b were used for immunohistochemistry. Morphology and distribution of astrocytes was evaluated under light microscope and their density was quantitatively analysed.

Results. In the CA1 hippocampal area of group II-28 rats, GFAP-IR cells with numerous, branched processes were observed. S100b-IR astrocytes had delicate, single processes in comparison with cells without processes observed in groups I-7 and C. In groups I-7 and II-28, GFAP-IR astrocytes’ density significantly increased in SR — stratum radiatum of hippocampal CA1 area. In group I-7, a density of cells with the expression of S100b was significantly increased in SO — stratum oriens layer. In group II-28, the density of S100b-IR astrocytes was decreased.

Conclusions. Habanero peppers administrated to rats, especially for a longer time, caused reactive changes in the astrocytes in hippocampal CA1 area, and thus these glial cells may protect neurons against excitotoxic damage.

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Keywords

rat; hippocampus; CA1; astrocytes; GFAP; S100b; capsaicin; IHC

About this article
Title

Reactivity of astrocytes in hippocampal CA1 area in rats after administration of habanero peppers

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 59, No 1 (2021)

Article type

Original paper

Pages

1-7

Published online

2021-01-27

Page views

1082

Article views/downloads

769

DOI

10.5603/FHC.a2021.0001

Pubmed

33502751

Bibliographic record

Folia Histochem Cytobiol 2021;59(1):1-7.

Keywords

rat
hippocampus
CA1
astrocytes
GFAP
S100b
capsaicin
IHC

Authors

Jadwiga Jaworska-Adamu
Aleksandra Krawczyk
Karol Rycerz
Marcin Golynski

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