open access

Vol 58, No 2 (2020)
ORIGINAL PAPERS
Published online: 2020-06-22
Submitted: 2019-03-06
Accepted: 2020-06-09
Get Citation

The effect of dental gel formulation on human primary fibroblasts — an in vitro study

Barbara Sterczala, Julita Kulbacka, Jolanta Saczko, Marzena Dominiak
DOI: 10.5603/FHC.a2020.0013
·
Pubmed: 32567043
·
Folia Histochem Cytobiol 2020;58(2):156-161.

open access

Vol 58, No 2 (2020)
ORIGINAL PAPERS
Published online: 2020-06-22
Submitted: 2019-03-06
Accepted: 2020-06-09

Abstract

Introduction. In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. The aim of our study was to evaluate the protective properties and the safety of Protefix® gel application after dental procedures in vitro. Material and methods. Human gingival fibroblasts (HGFs) were isolated from normal gingival tissues, cultured to full monolayer and exposed to Protefix® gel in the concentration from 1 to 100%. The viability of cells was examined by MTT assays. Cell migration as a response of treated cells was assessed. The expression of collagen III was estimated by immunocytochemistry after 20 minutes or 24 hours incubation with Protefix® gel. Results. The obtained results indicated that the verified gel significantly stimulated fibroblasts’ proliferation, and mitochondrial activity determined by MTT assay increased almost two-fold for lower gel concentrations. The immunohistochemical detection of collagen III revealed an increased expression after incubation with 5% gel.

Conclusions. The results proved that the gel is safe for cells derived from human gingiva and moreover has regenerative properties, which can be of great importance in the treatment of gingivitis after retraction and surgical procedures, or even ordinary daily injuries of oral cavity.

Abstract

Introduction. In ordinary dental practice, the dentist often meets with patients suffering from ulcers, aphtha with edema, bleeding gums, bothersome burning, and dry mouth. These are prosthetic, orthodontic patients, after surgery, in various age ranges. Protefix® gel is a soothing and regenerating preparation aimed at patients with mucosal problems. The aim of our study was to evaluate the protective properties and the safety of Protefix® gel application after dental procedures in vitro. Material and methods. Human gingival fibroblasts (HGFs) were isolated from normal gingival tissues, cultured to full monolayer and exposed to Protefix® gel in the concentration from 1 to 100%. The viability of cells was examined by MTT assays. Cell migration as a response of treated cells was assessed. The expression of collagen III was estimated by immunocytochemistry after 20 minutes or 24 hours incubation with Protefix® gel. Results. The obtained results indicated that the verified gel significantly stimulated fibroblasts’ proliferation, and mitochondrial activity determined by MTT assay increased almost two-fold for lower gel concentrations. The immunohistochemical detection of collagen III revealed an increased expression after incubation with 5% gel.

Conclusions. The results proved that the gel is safe for cells derived from human gingiva and moreover has regenerative properties, which can be of great importance in the treatment of gingivitis after retraction and surgical procedures, or even ordinary daily injuries of oral cavity.

Get Citation

Keywords

Protefix® gel; human gingival fibroblasts; cell proliferation; collagen III

About this article
Title

The effect of dental gel formulation on human primary fibroblasts — an in vitro study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 58, No 2 (2020)

Pages

156-161

Published online

2020-06-22

DOI

10.5603/FHC.a2020.0013

Pubmed

32567043

Bibliographic record

Folia Histochem Cytobiol 2020;58(2):156-161.

Keywords

Protefix® gel
human gingival fibroblasts
cell proliferation
collagen III

Authors

Barbara Sterczala
Julita Kulbacka
Jolanta Saczko
Marzena Dominiak

References (17)
  1. Yu GY. Oral and maxillofacial surgery: Current and future. Ann Maxillofac Surg. 2013; 3(2): 111–112.
  2. Wolf DL, Lamster IB. Contemporary concepts in the diagnosis of periodontal disease. Dent Clin North Am. 2011; 55(1): 47–61.
  3. Góra J, Lis A, Kula J, et al. Chemical composition variability of essential oils in the ontogenesis of some plants. Flavour and Fragrance Journal. 2002; 17(6): 445–451.
  4. Sterczała B, Kulbacka J, Saczko J, et al. : Evaluation of keratinized gingival fibroblast proliferation after Protefix® gel application, in the aspect of wound healing in elderly patients, J Stoma 2019; 72. ; 1: 8–11.
  5. Upadhay RK. : Plant natural products: Their pharmaceutical potential against disease and drug resistant microbial pathogens, J Pharm Res. 2011; 4(4): 1179–1185.
  6. Sakagami H, Tomomura M. Dental Application of Natural Products. Medicines (Basel). 2018; 5(1).
  7. Cimanga K, Kambu K, Tona L, et al. Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. J Ethnopharmacol. 2002; 79(2): 213–220.
  8. Park MJ, Gwak KS, Yang I, et al. Effect of citral, eugenol, nerolidol and alpha-terpineol on the ultrastructural changes of Trichophyton mentagrophytes. Fitoterapia. 2009; 80(5): 290–296.
  9. Omidbeygi M, Barzegar M, Hamidi Z, et al. Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control. 2007; 18(12): 1518–1523.
  10. Dominiak M, Łysiak-Drwal K, Saczko J, et al. A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation. Folia Histochem Cytobiol. 2008; 46(1): 117–119.
  11. Volk SW, Wang Y, Mauldin EA, et al. Diminished type III collagen promotes myofibroblast differentiation and increases scar deposition in cutaneous wound healing. Cells Tissues Organs. 2011; 194(1): 25–37.
  12. Drąg-Zalesińska M, Rembiałkowska N, Borska S, et al. A New Betulin Derivative Stimulates the Synthesis of Collagen in Human Fibroblasts Stronger than its Precursor. In Vivo. 2019; 33(4): 1087–1093.
  13. Jurge S, Kuffer R, Scully C, et al. Mucosal disease series. Number VI. Recurrent aphthous stomatitis. Oral Dis. 2006; 12(1): 1–21.
  14. Scully C, Porter S. Oral mucosal disease: Recurrent aphthous stomatitis. Br J Oral Maxillofac Surg. 2008; 46(3): 198–206.
  15. Baccaglini L, Lalla RV, Bruce AJ, et al. Urban legends: recurrent aphthous stomatitis. Oral Dis. 2011; 17(8): 755–770.
  16. Ofluoglu D, Ergun S, Warmakulasuiriya S, et al. : Triester Glycerol Oxide gel in the treatment of minor RAS, Med Oral Patol Oral Cir Bucal. 2017; 22(2): 159–66.
  17. Kumada Y, Zhang S. Significant type I and type III collagen production from human periodontal ligament fibroblasts in 3D peptide scaffolds without extra growth factors. PLoS One. 2010; 5(4): e10305.

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