open access

Vol 58, No 2 (2020)
ORIGINAL PAPERS
Published online: 2020-06-18
Submitted: 2019-12-25
Accepted: 2020-06-03
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Bisdemethoxycurcumin exerts a cell-protective effect via JAK2/STAT3 signaling in a rotenone-induced Parkinson’s disease model in vitro

Duanqun He, Shuangxi Chen, Zijian Xiao, Heng Wu, Guijuan Zhou, Chenlin Xu, Yunqian Chang, Yihui Li, Gang Wang, Ming Xie
DOI: 10.5603/FHC.a2020.0011
·
Pubmed: 32557525
·
Folia Histochem Cytobiol 2020;58(2):127-134.

open access

Vol 58, No 2 (2020)
ORIGINAL PAPERS
Published online: 2020-06-18
Submitted: 2019-12-25
Accepted: 2020-06-03

Abstract

Introduction. Oxidative stress and cell apoptosis have both been suggested to be closely associated with the pathogenesis of Parkinson’s disease (PD). Previously, bisdemethoxycurcumin (BDMC) has been shown to exhibit several desirable characteristics as a candidate neuroprotective agent, including antioxidant and anti-inflammatory activities in the nervous system. However, whether BDMC can exert cell-protective roles in an in vitro model of PD remains unknown.

Material and methods. SH-SY5Y cells were pretreated with BDMC, with or without AG490 and SI-201, for 30 min, followed by a co-incubation with rotenone for 24 h. Subsequently, a cell viability assay and western blotting was performed, and SOD and GSH activities were analyzed.

Results. The results revealed that the pretreatment with BDMC enhanced the cell survival, antioxidative stress capacity and the phosphorylation levels of JAK/STAT3 in SH-SY5Y cells treated with rotenone. However, following the incubation with AG490 and SI-201, inhibitors of the JAK/STAT3 signaling pathway, BDMC was unable to exert cell-protective roles in SH-SY5Y cells treated with rotenone.

Conclusions. In conclusion, the results suggested that BDMC may exert a cell-protective role in SH-SY5Y cells in vitro via JAK2/STAT3 signaling, thus suggesting the possible application of BDMC for the treatment of neurodegenerative diseases related to JAK2/STAT3 signaling.

Abstract

Introduction. Oxidative stress and cell apoptosis have both been suggested to be closely associated with the pathogenesis of Parkinson’s disease (PD). Previously, bisdemethoxycurcumin (BDMC) has been shown to exhibit several desirable characteristics as a candidate neuroprotective agent, including antioxidant and anti-inflammatory activities in the nervous system. However, whether BDMC can exert cell-protective roles in an in vitro model of PD remains unknown.

Material and methods. SH-SY5Y cells were pretreated with BDMC, with or without AG490 and SI-201, for 30 min, followed by a co-incubation with rotenone for 24 h. Subsequently, a cell viability assay and western blotting was performed, and SOD and GSH activities were analyzed.

Results. The results revealed that the pretreatment with BDMC enhanced the cell survival, antioxidative stress capacity and the phosphorylation levels of JAK/STAT3 in SH-SY5Y cells treated with rotenone. However, following the incubation with AG490 and SI-201, inhibitors of the JAK/STAT3 signaling pathway, BDMC was unable to exert cell-protective roles in SH-SY5Y cells treated with rotenone.

Conclusions. In conclusion, the results suggested that BDMC may exert a cell-protective role in SH-SY5Y cells in vitro via JAK2/STAT3 signaling, thus suggesting the possible application of BDMC for the treatment of neurodegenerative diseases related to JAK2/STAT3 signaling.

Get Citation

Keywords

oxidative stress; rotenone; SH-SY5Y cells; bisdemethoxycurcumin (BDMC); JAK/STAT3

About this article
Title

Bisdemethoxycurcumin exerts a cell-protective effect via JAK2/STAT3 signaling in a rotenone-induced Parkinson’s disease model in vitro

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 58, No 2 (2020)

Pages

127-134

Published online

2020-06-18

DOI

10.5603/FHC.a2020.0011

Pubmed

32557525

Bibliographic record

Folia Histochem Cytobiol 2020;58(2):127-134.

Keywords

oxidative stress
rotenone
SH-SY5Y cells
bisdemethoxycurcumin (BDMC)
JAK/STAT3

Authors

Duanqun He
Shuangxi Chen
Zijian Xiao
Heng Wu
Guijuan Zhou
Chenlin Xu
Yunqian Chang
Yihui Li
Gang Wang
Ming Xie

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