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Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary
Affiliations- Biotech Research & Innovation Centre, University of Copenhagen, Copenhagen, Denmark
open access
Abstract
Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.
Abstract
Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.
Keywords
TGF-β; latent TGF-binding protein; cell free ECM; high pH buffers
About this articleTitle
Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Commentary
Pages
157-158
Published online
2019-11-19
Page views
1499
Article views/downloads
768
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2019;57(4):157-158.
Keywords
TGF-β
latent TGF-binding protein
cell free ECM
high pH buffers
Authors
John R. Couchman
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