open access

Vol 57, No 3 (2019)
ORIGINAL PAPERS
Published online: 2019-09-25
Submitted: 2019-09-04
Accepted: 2019-09-23
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Diagnostic immunohistochemistry for canine cutaneous round cell tumours — retrospective analysis of 60 cases

Katarzyna Pazdzior-Czapula, Mateusz Mikiewicz, Michal Gesek, Cezary Zwolinski, Iwona Otrocka-Domagala
DOI: 10.5603/FHC.a2019.0016
·
Pubmed: 31553052
·
Folia Histochem Cytobiol 2019;57(3):146-154.

open access

Vol 57, No 3 (2019)
ORIGINAL PAPERS
Published online: 2019-09-25
Submitted: 2019-09-04
Accepted: 2019-09-23

Abstract

Introduction. Canine cutaneous round cell tumours (CCRCTs) include various benign and malignant neoplastic processes. Due to their similar morphology, the diagnosis of CCRCTs based on histopathological examination alone can be challenging, often necessitating ancillary immunohistochemical (IHC) analysis. This study presents a retrospective analysis of CCRCTs.

Materials and methods. This study includes 60 cases of CCRCTs, including 55 solitary and 5 multiple tumours, evaluated immunohistochemically using a basic antibody panel (MHCII, CD18, Iba1, CD3, CD79a, CD20 and mast cell tryptase) and, when appropriate, extended antibody panel (vimentin, desmin, a-SMA, S-100, melan-A and pan-keratin). Additionally, histochemical stainings (May-Grünwald-Giemsa and methyl green pyronine) were performed.

Results. IHC analysis using a basic antibody panel revealed 27 cases of histiocytoma, one case of histiocytic sarcoma, 18 cases of cutaneous lymphoma of either T-cell (CD3+) or B-cell (CD79a+) origin, 5 cases of plas­macytoma, and 4 cases of mast cell tumours. The extended antibody panel revealed 2 cases of alveolar rhabdo­myosarcoma, 2 cases of amelanotic melanoma, and one case of glomus tumour.

Conclusions. Both canine cutaneous histiocytoma and cutaneous lymphoma should be considered at the beginning of differential diagnosis for CCRCTs. While most poorly differentiated CCRCTs can be diagnosed immunohis­tochemically using 1–4 basic antibodies, some require a broad antibody panel, including mesenchymal, epithelial, myogenic, and melanocytic markers. The expression of Iba1 is specific for canine cutaneous histiocytic tumours, and more sensitive than CD18. The utility of CD20 in the diagnosis of CCRCTs is limited.

 

Abstract

Introduction. Canine cutaneous round cell tumours (CCRCTs) include various benign and malignant neoplastic processes. Due to their similar morphology, the diagnosis of CCRCTs based on histopathological examination alone can be challenging, often necessitating ancillary immunohistochemical (IHC) analysis. This study presents a retrospective analysis of CCRCTs.

Materials and methods. This study includes 60 cases of CCRCTs, including 55 solitary and 5 multiple tumours, evaluated immunohistochemically using a basic antibody panel (MHCII, CD18, Iba1, CD3, CD79a, CD20 and mast cell tryptase) and, when appropriate, extended antibody panel (vimentin, desmin, a-SMA, S-100, melan-A and pan-keratin). Additionally, histochemical stainings (May-Grünwald-Giemsa and methyl green pyronine) were performed.

Results. IHC analysis using a basic antibody panel revealed 27 cases of histiocytoma, one case of histiocytic sarcoma, 18 cases of cutaneous lymphoma of either T-cell (CD3+) or B-cell (CD79a+) origin, 5 cases of plas­macytoma, and 4 cases of mast cell tumours. The extended antibody panel revealed 2 cases of alveolar rhabdo­myosarcoma, 2 cases of amelanotic melanoma, and one case of glomus tumour.

Conclusions. Both canine cutaneous histiocytoma and cutaneous lymphoma should be considered at the beginning of differential diagnosis for CCRCTs. While most poorly differentiated CCRCTs can be diagnosed immunohis­tochemically using 1–4 basic antibodies, some require a broad antibody panel, including mesenchymal, epithelial, myogenic, and melanocytic markers. The expression of Iba1 is specific for canine cutaneous histiocytic tumours, and more sensitive than CD18. The utility of CD20 in the diagnosis of CCRCTs is limited.

 

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Keywords

canine cutaneous tumours; IHC diagnosis; histiocytoma; cutaneous lymphoma; plasmacytoma; mast cell tumour; alveolar rhabdomyosarcoma, amelanotic melanoma, glomus tumour

About this article
Title

Diagnostic immunohistochemistry for canine cutaneous round cell tumours — retrospective analysis of 60 cases

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 57, No 3 (2019)

Pages

146-154

Published online

2019-09-25

DOI

10.5603/FHC.a2019.0016

Pubmed

31553052

Bibliographic record

Folia Histochem Cytobiol 2019;57(3):146-154.

Keywords

canine cutaneous tumours
IHC diagnosis
histiocytoma
cutaneous lymphoma
plasmacytoma
mast cell tumour
alveolar rhabdomyosarcoma
amelanotic melanoma
glomus tumour

Authors

Katarzyna Pazdzior-Czapula
Mateusz Mikiewicz
Michal Gesek
Cezary Zwolinski
Iwona Otrocka-Domagala

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