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Gallbladder interstitial Cajal-like cells and gallbladder contractility in patients with cholelithiasis: a prospective study
Affiliations- National Center of Gerontology, Beijing Hospital, Beijing, China
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Abstract
Introduction. A reduced number of interstitial Cajal-like cells (ICLCs) in the gallbladder have been proposed to play a role in the pathogenesis of cholelithiasis. Therefore, this prospective study was conducted to investigate the relationship between gallbladder contractility and the number of gallbladder ICLCs in patients with cholelithiasis.
Material and methods. Patients admitted to the Department of Hepatobiliary Surgery for cholecystectomy were divided into the cholelithiasis (n = 18) and non-cholelithiasis (n = 8) groups based on their clinical data. Patients’ clinical data were collected on admission, and B-mode ultrasonography was performed to assess their gallbladder contractility. The resected gallbladder specimens were fixed, paraffin sections mounted on slides, and the immunofluorescence staining with the anti-human CD-117 and anti-human tryptase antibodies was performed to identify ICLSs and mast cells, respectively. The number of ICLCs was counted in 10 high-power fields (HPFs) randomly.
Results. Independent sample t-tests revealed differences between the cholelithiasis and non-cholelithiasis groups in the number of ICLCs (mean ± standard deviation: 88.61 ± 28.22 vs. 115.89 ± 27.87 per HPFs, P = 0.032) and gallbladder contractility (43.94% ± 18.50% vs. 61.00% ± 20.50%, P = 0.046). Pearson and Spearman correlation analyses revealed no significant correlation between the number of ICLCs and gallbladder contractility.
Conclusion. The results suggest that the number of gallbladder ICLCs in the wall of the gallbladder of patients with or without cholelithiasis is not a decisive factor affecting gallbladder contractility.
Abstract
Introduction. A reduced number of interstitial Cajal-like cells (ICLCs) in the gallbladder have been proposed to play a role in the pathogenesis of cholelithiasis. Therefore, this prospective study was conducted to investigate the relationship between gallbladder contractility and the number of gallbladder ICLCs in patients with cholelithiasis.
Material and methods. Patients admitted to the Department of Hepatobiliary Surgery for cholecystectomy were divided into the cholelithiasis (n = 18) and non-cholelithiasis (n = 8) groups based on their clinical data. Patients’ clinical data were collected on admission, and B-mode ultrasonography was performed to assess their gallbladder contractility. The resected gallbladder specimens were fixed, paraffin sections mounted on slides, and the immunofluorescence staining with the anti-human CD-117 and anti-human tryptase antibodies was performed to identify ICLSs and mast cells, respectively. The number of ICLCs was counted in 10 high-power fields (HPFs) randomly.
Results. Independent sample t-tests revealed differences between the cholelithiasis and non-cholelithiasis groups in the number of ICLCs (mean ± standard deviation: 88.61 ± 28.22 vs. 115.89 ± 27.87 per HPFs, P = 0.032) and gallbladder contractility (43.94% ± 18.50% vs. 61.00% ± 20.50%, P = 0.046). Pearson and Spearman correlation analyses revealed no significant correlation between the number of ICLCs and gallbladder contractility.
Conclusion. The results suggest that the number of gallbladder ICLCs in the wall of the gallbladder of patients with or without cholelithiasis is not a decisive factor affecting gallbladder contractility.
Keywords
cholelithiasis; interstitial Cajal-like cells; mast cells; gallbladder contractility; immunofluorescence; ultrasonography
About this articleTitle
Gallbladder interstitial Cajal-like cells and gallbladder contractility in patients with cholelithiasis: a prospective study
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
94-100
Published online
2019-06-24
Page views
1867
Article views/downloads
979
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2019;57(2):94-100.
Keywords
cholelithiasis
interstitial Cajal-like cells
mast cells
gallbladder contractility
immunofluorescence
ultrasonography
Authors
Runyu Ding
Junmin Wei
Jingyong Xu
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