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Immunohistochemical characteristic of C cells in European bison thyroid gland
Affiliations- Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland
- Departament of Small Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159c,, 02-776 Warsaw, Poland
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Abstract
Introduction. C cells constitute a small percentage of thyroid gland parenchyma. The number, morphology and distribution of C cells differ among species; however, data regarding their characteristics in European bison are sparse. The aim of this study was to evaluate the morphology, distribution pattern and percentage of C cells in European bison thyroid gland together with morphometric analysis.
Material and methods. Thyroid glands from 28 European bisons of different sex and age were collected either in autumn-winter (13/28) or in spring-summer (15/28) periods and analyzed by immunohistochemistry.
Results. The mean total C cell number per all endocrine (follicular and C cells) cell number (C cell concentration) was 7.33%. The tendency to increase the C cell number from periphery to the central region of thyroid lobe was observed with the mean C cell concentration of 3.95%, 7.89% and 9.97% in peripheral, intermediate and central areas, respectively. Most frequently, C cells were situated intrafolliculary whereas epifollicular and interfollicular positions were observed less often. C cells were polymorphic with long cytoplasmic processes. The mean C cell area was 61.97 μm2 and the mean C cell perimeter, length and width were: 34.92 μm, 12.85 μm and 4.91 μm, respectively. In the majority of C cells, strong immunohistochemical cytoplasmic reaction was observed with the mean color intensity of 78.32. In autumn-winter period, C cells were significantly larger with lower color intensity than during spring and summer.
Conclusions. This study leads to deeper characteristics of thyroid gland C cells in European bison. The histomorphometric data suggest that in European bison production of calcitonin by C cells may differ depending on the time of the year.
Abstract
Introduction. C cells constitute a small percentage of thyroid gland parenchyma. The number, morphology and distribution of C cells differ among species; however, data regarding their characteristics in European bison are sparse. The aim of this study was to evaluate the morphology, distribution pattern and percentage of C cells in European bison thyroid gland together with morphometric analysis.
Material and methods. Thyroid glands from 28 European bisons of different sex and age were collected either in autumn-winter (13/28) or in spring-summer (15/28) periods and analyzed by immunohistochemistry.
Results. The mean total C cell number per all endocrine (follicular and C cells) cell number (C cell concentration) was 7.33%. The tendency to increase the C cell number from periphery to the central region of thyroid lobe was observed with the mean C cell concentration of 3.95%, 7.89% and 9.97% in peripheral, intermediate and central areas, respectively. Most frequently, C cells were situated intrafolliculary whereas epifollicular and interfollicular positions were observed less often. C cells were polymorphic with long cytoplasmic processes. The mean C cell area was 61.97 μm2 and the mean C cell perimeter, length and width were: 34.92 μm, 12.85 μm and 4.91 μm, respectively. In the majority of C cells, strong immunohistochemical cytoplasmic reaction was observed with the mean color intensity of 78.32. In autumn-winter period, C cells were significantly larger with lower color intensity than during spring and summer.
Conclusions. This study leads to deeper characteristics of thyroid gland C cells in European bison. The histomorphometric data suggest that in European bison production of calcitonin by C cells may differ depending on the time of the year.
Keywords
C cells; European bison; histology; immunohistochemistry; morphometry; thyroid gland
About this articleTitle
Immunohistochemical characteristic of C cells in European bison thyroid gland
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
222-230
Published online
2018-12-20
Page views
2881
Article views/downloads
1643
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2018;56(4):222-230.
Keywords
C cells
European bison
histology
immunohistochemistry
morphometry
thyroid gland
Authors
Justyna Sokolowska
Joanna Berczynska
Aleksandra Poweska
Dorota Rygiel
Katarzyna Olbrych
Kaja Urbanska
References (30)- Kupczyńska M, Barszcz K, Olbrych K, et al. Coronary arteries of the European bison (Bison bonasus). Acta Vet Scand. 2015; 57: 82.
- Osińska B, Demiaszkiewicz AW, Lachowicz J. Pathological lesions in European bison (Bison bonasus) with infestation by Ashworthius sidemi (Nematoda, Trichostrongylidae). Pol J Vet Sci. 2010; 13(1): 63–67.
- Czykier E, Krasińska M. Cases of spermiogenesis in young European bison. Acta Theriologica. 2004; 49(4): 543–554.
- Barszcz K, Przespolewska H, Olbrych K, et al. The morphology of the adrenal gland in the European bison (Bison bonasus). BMC Vet Res. 2016; 12(1): 161.
- Sawicki B, Siuda S, Kasacka I. Bisoniana 107. Microscopic structure of the thyroid gland in the European bison. Acta Theriologica. 1992; 37: 171–179.
- Sawicki B. Bisoniana 106. Ultimobranchial follicles and cysts in the European bison thyroid. Acta Theriologica. 1991; 36: 349–356.
- Mara J, Morillo-Bernal J, Garca-Marn R, et al. Paracrine Regulation of Thyroid-Hormone Synthesis by C Cells. Thyroid Hormone. 2012.
- Martín-Lacave I, Conde E, Montero C, et al. Quantitative changes in the frequency and distribution of the C-cell population in the rat thyroid gland with age. Cell Tissue Res. 1992; 270(1): 73–77.
- Sawicki B. Morphology and histochemistry of thyroid gland C cells of young and adult guinea pigs. Acta Theriologica. 1975; 20: 281-296, plates 5–7.
- Wolfe HJ, Voelkel EF, Tashjian AH. Distribution of calcitonin-containing cells in the normal adult human thyroid gland: a correlation of morphology with peptide content. J Clin Endocrinol Metab. 1974; 38(4): 688–694.
- Sawicki B, Kuczyński M. Morphological studies on the C cells of the thyroid of certain rodents. Acta Theriologica. 1977; 22: 251-260, plates 16–17.
- Zabel M, Schäfer H, Surdyk J, et al. Immunocytochemical studies on parafollicular cells of various mammals. Acta Anat (Basel). 1988; 131(3): 222–226.
- Kameda Y. Parafollicular cells of the thyroid as studied with Davenport's silver impregnation. Arch Histol Jpn. 1968; 30(1): 83–94.
- Tsuchiya T, Shiomura Y, Suzuki K, et al. Immunocytochemical study on the C cells in pig thyroid glands. Acta Anat (Basel). 1984; 120(3): 138–141.
- Sawicki B, Zabel M. Immunocytochemical study of parafollicular cells of the thyroid and ultimobranchial remnants of the European bison. Acta Histochem. 1997; 99(2): 223–230.
- Sawicki B. C-Zellen in der Schilddrüse und in den Resten des Ultimobranchialkörpers des Wisents. Annals of Anatomy - Anatomischer Anzeiger. 1993; 175(4): 343–347.
- Ljungberg O, Nilsson PO. Hyperplastic and neoplastic changes in ultimobranchial remnants and in parafollicular (C) cells in bulls: a histologic and immunohistochemical study. Vet Pathol. 1985; 22(2): 95–103.
- Collins WT, Capen CC, Döbereiner J, et al. Ultrastructural evaluation of parathyroid glands and thyroid C cells of cattle fed Solanum malacoxylon. Am J Pathol. 1977; 87(3): 603–614.
- Perry A, Molberg K, Albores-Saavedra J. Physiologic versus neoplastic C-cell hyperplasia of the thyroid: separation of distinct histologic and biologic entities. Cancer. 1996; 77(4): 750–756.
- Sakorafas GH, Nasikas D, Thanos D, et al. Incidental thyroid C cell hyperplasia: clinical significance and implications in practice. Oncol Res Treat. 2015; 38(5): 249–252.
- Cameselle-Teijeiro J, Varela-Durán J, Sambade C, et al. Solid cell nests of the thyroid: light microscopy and immunohistochemical profile. Hum Pathol. 1994; 25(7): 684–693.
- Mizukami Y, Nonomura A, Michigishi T, et al. Solid cell nests of the thyroid. A histologic and immunohistochemical study. Am J Clin Pathol. 1994; 101(2): 186–191.
- Shubbur A, Goffaux M, Thibier M. Evolution saisonnière des niveaux plasmatiques de thyroxine et de triiodothyronine chez le taureau post-pubère en France et en Irak. Variations concomitantes des taux de LH et de testostérone. Reproduction Nutrition Développement. 1989; 29(3): 309–315.
- Hamr J, Bubenik GA. Seasonal thyroid hormone levels of free-ranging white-tailed deer (Odocoileus virginianus) in Ontario. Canadian Journal of Zoology. 1990; 68(10): 2174–2180.
- Frink R, Krupp PP, Young RA. Seasonal variation in the morphology of thyroid parafollicular (C) cells in the woodchuck, Marmota monax: a light and electron microscopic study. Anat Rec. 1977; 189(3): 397–411.
- Nunez EA, Gould RP, Holt SJ. Seasonal changes in secretory granules and crystalloid inclusions of bat thyroid parafollicular cells. J Cell Sci. 1970; 6(3): 821–841.
- Smith BB, Van Saun RJ. Seasonal changes in serum calcium, phosphorus, and vitamin D concentrations in llamas and alpacas. Am J Vet Res. 2001; 62(8): 1187–1193.
- Nevo-Shor A, Kogan S, Joshua BZ, et al. Seasonal changes in serum calcium, PTH and vitamin D levels in patients with primary hyperparathyroidism. Bone. 2016; 89: 59–63.
- Chao CC, Brown RD, Deftos LJ. Seasonal levels of serum parathyroid hormone, calcitonin and alkaline phosphatase in relation to antler cycles in white-tailed deer. Acta Endocrinol (Copenh). 1984; 106(2): 234–240.
- Kuba J, Błaszczyk B, Stankiewicz T, et al. Circannual changes in serum concentrations of thyroxine, calcitonin and parathormone in immature and mature red deer females (Cervus elaphus). Pol J Vet Sci. 2015; 18(3): 613–619.
