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Vol 56, No 2 (2018)
Original paper
Submitted: 2018-04-19
Accepted: 2018-05-29
Published online: 2018-06-04
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Immunohistochemical study on neuropilin 1 (NRP1) immunoexpression in oral squamous cell carcinoma

Olga Stasikowska-Kanicka1, Małgorzata Wągrowska-Danilewicz1, Marian Danilewicz2
DOI: 10.5603/FHC.a2018.0010
·
Pubmed: 29873058
·
Folia Histochem Cytobiol 2018;56(2):98-105.
Affiliations
  1. Department of Nephropathology, Medical University of Lodz, Lodz, Poland
  2. Department of Pathomorphology, Medical University of Lodz, Lodz, Poland

open access

Vol 56, No 2 (2018)
ORIGINAL PAPERS
Submitted: 2018-04-19
Accepted: 2018-05-29
Published online: 2018-06-04

Abstract

Introduction. Neuropilins (NRPs) are multifunctional glycoproteins that play an important role in angiogenesis and cancer progression. The aim of the study was to examine the immunoexpression of neuropilin 1 (NRP1), the number of NRP1+ infiltrating cells and CD163+ macrophages, and density of microvessels (MVD) in oral squamous cell carcinoma (OSCC).

Material and methods. The study was performed on 45 OSCC patients with metastases (OSCCM+), 51 patients without metastases (OSCCM-) and 17 control cases. The microvessels were identified by the presence of CD31 and the expression of the studied proteins was assessed by immunohistochemistry.

Results. The immunoexpression of NRP1, the mean numbers of NRP1+, CD163+ infiltrating cells, and MVD were significantly increased in OSCCM+ patients in comparison to OSCCM-, and control groups. Moreover, in OSCCM- patients all these parameters were also significantly increased in comparison to controls. In OSCCM+ and OSCCM- groups, there were positive correlations between the immunoexpression of NRP1 and MVD (r = 0.41, p < 0.006; r = 0.51, p < 0.001, respectively), and between the number of NRP1+ infiltrating cells and CD163+ macrophages (r = 0.56, p < 0.001, r = 0.49, p < 0.001, respectively).

Conclusions. The present study revealed overexpression of NRP1 in OSCC, especially in OSCC patients with metasta­sis, suggesting that NRP1 could potentially contribute to metastasis of oral cancer. The correlation between the number of NRP1+ infiltrating cells and CD163+ macrophages suggests that NRP1+ infiltrating macrophages are present in tumor microenvironment and may play a role in the progressions of oral cancer.

Abstract

Introduction. Neuropilins (NRPs) are multifunctional glycoproteins that play an important role in angiogenesis and cancer progression. The aim of the study was to examine the immunoexpression of neuropilin 1 (NRP1), the number of NRP1+ infiltrating cells and CD163+ macrophages, and density of microvessels (MVD) in oral squamous cell carcinoma (OSCC).

Material and methods. The study was performed on 45 OSCC patients with metastases (OSCCM+), 51 patients without metastases (OSCCM-) and 17 control cases. The microvessels were identified by the presence of CD31 and the expression of the studied proteins was assessed by immunohistochemistry.

Results. The immunoexpression of NRP1, the mean numbers of NRP1+, CD163+ infiltrating cells, and MVD were significantly increased in OSCCM+ patients in comparison to OSCCM-, and control groups. Moreover, in OSCCM- patients all these parameters were also significantly increased in comparison to controls. In OSCCM+ and OSCCM- groups, there were positive correlations between the immunoexpression of NRP1 and MVD (r = 0.41, p < 0.006; r = 0.51, p < 0.001, respectively), and between the number of NRP1+ infiltrating cells and CD163+ macrophages (r = 0.56, p < 0.001, r = 0.49, p < 0.001, respectively).

Conclusions. The present study revealed overexpression of NRP1 in OSCC, especially in OSCC patients with metasta­sis, suggesting that NRP1 could potentially contribute to metastasis of oral cancer. The correlation between the number of NRP1+ infiltrating cells and CD163+ macrophages suggests that NRP1+ infiltrating macrophages are present in tumor microenvironment and may play a role in the progressions of oral cancer.

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Keywords

neuropilin 1; macrophages; angiogenesis; oral cancer; metastasis; IHC

About this article
Title

Immunohistochemical study on neuropilin 1 (NRP1) immunoexpression in oral squamous cell carcinoma

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 56, No 2 (2018)

Article type

Original paper

Pages

98-105

Published online

2018-06-04

Page views

1499

Article views/downloads

1498

DOI

10.5603/FHC.a2018.0010

Pubmed

29873058

Bibliographic record

Folia Histochem Cytobiol 2018;56(2):98-105.

Keywords

neuropilin 1
macrophages
angiogenesis
oral cancer
metastasis
IHC

Authors

Olga Stasikowska-Kanicka
Małgorzata Wągrowska-Danilewicz
Marian Danilewicz

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