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Caspase-3 as an important factor in the early cytotoxic effect of nickel on oral mucosa cells in patients treated orthodontically
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Abstract
Introduction. The effect of fixed orthodontic appliances on biochemical changes in saliva and pathophysiological status of the oral cavity is not clear. Recent data showed that nickel (Ni) released from orthodontic appliances can decrease cellular viability, induce DNA damage and apoptosis in oral mucosa cells. Since the mechanism of these Ni effects is unknown, the aim of our study was to analyze the expression of caspase-3 in epithelial cells of oral mucosa in healthy individuals treated orthodontically.
Material and methods. Twenty-eight volunteers participated in the study. Epithelial cells were collected from oral mucosa directly before appliance insertion, one week after the insertion, and 24 four weeks after the insertion of fixed appliances. Cellular identification and measurements were conducted by light microscopy. Caspase-3 expression was evaluated immunochemically. Nickel concentration in saliva was also determined.
Results. A significantly higher number of oral epithelial cells with caspase-3 immunoreactivity in was found one week, but not 24 weeks, after orthodontic treatment. The enhanced expression of caspase-3 was accompanied by increased nickel concentration in saliva.
Conclusions. Our data suggests that nickel released from orthodontic appliances can activate caspase-3 and this mechanism may be partially responsible for the cytotoxic action of nickel in the oral cavity of orthodontically-treated individuals.
Abstract
Introduction. The effect of fixed orthodontic appliances on biochemical changes in saliva and pathophysiological status of the oral cavity is not clear. Recent data showed that nickel (Ni) released from orthodontic appliances can decrease cellular viability, induce DNA damage and apoptosis in oral mucosa cells. Since the mechanism of these Ni effects is unknown, the aim of our study was to analyze the expression of caspase-3 in epithelial cells of oral mucosa in healthy individuals treated orthodontically.
Material and methods. Twenty-eight volunteers participated in the study. Epithelial cells were collected from oral mucosa directly before appliance insertion, one week after the insertion, and 24 four weeks after the insertion of fixed appliances. Cellular identification and measurements were conducted by light microscopy. Caspase-3 expression was evaluated immunochemically. Nickel concentration in saliva was also determined.
Results. A significantly higher number of oral epithelial cells with caspase-3 immunoreactivity in was found one week, but not 24 weeks, after orthodontic treatment. The enhanced expression of caspase-3 was accompanied by increased nickel concentration in saliva.
Conclusions. Our data suggests that nickel released from orthodontic appliances can activate caspase-3 and this mechanism may be partially responsible for the cytotoxic action of nickel in the oral cavity of orthodontically-treated individuals.
Keywords
orthodontic appliances; epithelial oral mucosa cells; caspase-3; nickel; saliva; IHC
About this articleTitle
Caspase-3 as an important factor in the early cytotoxic effect of nickel on oral mucosa cells in patients treated orthodontically
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
37-42
Published online
2017-04-28
Page views
1282
Article views/downloads
1622
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2017;55(1):37-42.
Keywords
orthodontic appliances
epithelial oral mucosa cells
caspase-3
nickel
saliva
IHC
Authors
Piotr Buczko
Izabela Szarmach
Monika Grycz
Irena Kasacka
References (21)- Soesetijo FXA, Darmanto W, Prijatmoko D, et al. The effect of nickel as product of recasting nickel chromium on gingival fibroblasts apoptosis. J Int Dent Med Res. 2015; 8(1): 25–30.
- Craig RG, Power JM. Restorative Dental Materials, 11th ed. Mosby Inc, St Louis Missouri 2002.
- Gölz L, Knickenberg AC, Keilig L, et al. Nickel ion concentrations in the saliva of patients treated with self-ligating fixed appliances: a prospective cohort study. J Orofac Orthop. 2016; 77(2): 85–93.
- Hussain HD, Ajith SD, Goel P. Nickel release from stainless steel and nickel titanium archwires - An in vitro study. J Oral Biol Craniofac Res. 2016; 6(3): 213–218.
- Fariborz A, Alireza J, Parviz A, et al. Metal ion release from fixed orthodontic appliances--an in vivo study. Eur J Orthod. 2012; 34(1): 126–130.
- Faccioni F, Franceschetti P, Cerpelloni M, et al. In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells. Am J Orthod Dentofacial Orthop. 2003; 124(6): 687–93; discussion 693.
- Hafez HS, Selim EM, Kamel Eid FH, et al. Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: a longitudinal in-vivo study. Am J Orthod Dentofacial Orthop. 2011; 140(3): 298–308.
- Almeida A. Genetic determinants of neuronal vulnerability to apoptosis. Cell Mol Life Sci. 2013; 70(1): 71–88.
- Yu J, Zhao F, Wen X, et al. Apoptosis mechanism of gingival fibroblasts induced by nickel ion contained in dental cast alloys. Biomed Mater Eng. 2012; 22(1-3): 151–157.
- Shi T, Qin T, Zhan D. Evaluation of biocompatibility of the medical nickel-free stainless steel by detecting expression of caspase-3. Oral Biomed. 2010; 03: 010.
- Herman GE, Elfont EA. The taming of immunohistochemistry: the new era of quality control. Biotech Histochem. 1991; 66(4): 194–199.
- Kocadereli L, Ataç PA, Kale PS, et al. Salivary nickel and chromium in patients with fixed orthodontic appliances. Angle Orthod. 2000; 70(6): 431–434, doi: 10.1043/0003-3219(2000)070<0431:SNACIP>2.0.CO;2.
- Buczko P, Knaś M, Szarmach I, et al. The impact of orthodontic treatment on antioxidant capacity of saliva. Adv Med Sci. 2016.
- Latvala S, Hedberg J, Di Bucchianico S, et al. Nickel Release, ROS Generation and Toxicity of Ni and NiO Micro- and Nanoparticles. PLoS One. 2016; 11(7): e0159684.
- Natarajan M, Padmanabhan S, Chitharanjan A, et al. Evaluation of the genotoxic effects of fixed appliances on oral mucosal cells and the relationship to nickel and chromium concentrations: an in-vivo study. Am J Orthod Dentofacial Orthop. 2011; 140(3): 383–388.
- Yao H, Tang X, Shao X, et al. Parthenolide protects human lens epithelial cells from oxidative stress-induced apoptosis via inhibition of activation of caspase-3 and caspase-9. Cell Res. 2007; 17(6): 565–571.
- Carvour M, Song C, Kaul S, et al. Chronic low-dose oxidative stress induces caspase-3-dependent PKCdelta proteolytic activation and apoptosis in a cell culture model of dopaminergic neurodegeneration. Ann N Y Acad Sci. 2008; 1139: 197–205.
- Kalia S, Bansal MP. Regulation of apoptosis by Caspases under oxidative stress conditions in mice testicular cells: in vitro molecular mechanism. Mol Cell Biochem. 2009; 322(1-2): 43–52.
- Savitskaya MA, Onishchenko GE, Savitskaya MA, et al. Mechanisms of Apoptosis. Biochemistry (Mosc). 2015; 80(11): 1393–1405.
- Labbé D, Teranishi MA, Hess A, et al. Activation of caspase-3 is associated with oxidative stress in the hydropic guinea pig cochlea. Hear Res. 2005; 202(1-2): 21–27.
- Tan SN, Sim SP, Khoo ASB. Potential role of oxidative stress-induced apoptosis in mediating chromosomal rearrangements in nasopharyngeal carcinoma. Cell Biosci. 2016; 6: 35.
