Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Abstract

Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.

Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10^–8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.

Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2–3 long (50–100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.

Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.