open access

Vol 53, No 1 (2015)
ORIGINAL PAPERS
Published online: 2015-04-14
Submitted: 2014-09-25
Accepted: 2015-03-09
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Ghrelin and obestatin in thyroid gland — immunohistochemical expression in nodular goiter, papillary and medullary cancer

Edyta Gurgul, Aldona Kasprzak, Agata Blaszczyk, Maciej Biczysko, Joanna Surdyk-Zasada, Agnieszka Seraszek-Jaros, Marek Ruchala
DOI: 10.5603/FHC.a2015.0004
·
Pubmed: 25765090
·
Folia Histochem Cytobiol 2015;53(1):19-25.

open access

Vol 53, No 1 (2015)
ORIGINAL PAPERS
Published online: 2015-04-14
Submitted: 2014-09-25
Accepted: 2015-03-09

Abstract

Introduction. Previous studies analyzing ghrelin and obestatin expression in thyroid gland tissue are not unanimous and are mostly related to ghrelin. The role of ghrelin and obestatin in the thyroid gland appears very interesting due to their probable involvement in cell proliferation. Furthermore, since the thyroid gland is associated with the maintenance of energy balance, the relationship between ghrelin, obestatin and thyroid function is worthy of consideration. The aim of the study was to assess ghrelin and obestatin immunocytochemical expression in nodular goiter (NG), papillary cancer (PTC) and medullary cancer (MTC).

Material and methods. Analyzed samples included 9 cases of NG, 8 cases of PTC and 11 cases of MTC. The analysis of ghrelin and obestatin expression was performed by use of the immunohistochemical (IHC) EnVision system and evaluated with filter HSV software (quantitative morphometric analysis).

Results. Quantitative ghrelin expression in MTC cells was higher than in NG (p = 0.013) and correlated negatively with the size of the tumor (r= –0.829, p < 0.05). We did not observe any differences in ghrelin expression neither between MTC and PTC nor between NG and PTC. Obestatin immunoexpression pattern in all analyzed specimens was irregular and poorly accented. The strongest immunoreactivity for obestatin was demonstrated in NG. In MTC obestatin expression was significantly weaker than in NG and PTC (p < 0.05 in both cases). In NG the intensity of obestatin immunostaining was significantly higher than that of ghrelin (p = 0.03). Conversely, ghrelin expression in MTC was definitely more evident than obestatin immunoreactivity (p < 0.01). There was no statistically significant difference between ghrelin and obestatin expression in PTC. No correlations were detected between reciprocal tissue expressions of ghrelin and obestatin in the analyzed specimens of NG, PTC or MTC.

Conclusions. The differences between ghrelin expression in NG and MTC suggest that ghrelin may be involved in thyroid cell proliferation. The differences between ghrelin and obestatin immunoreactivity in benign and malignant thyroid tumors could support the theory of alternative transcription of the preproghrelin gene and independent production of ghrelin and obestatin.

Abstract

Introduction. Previous studies analyzing ghrelin and obestatin expression in thyroid gland tissue are not unanimous and are mostly related to ghrelin. The role of ghrelin and obestatin in the thyroid gland appears very interesting due to their probable involvement in cell proliferation. Furthermore, since the thyroid gland is associated with the maintenance of energy balance, the relationship between ghrelin, obestatin and thyroid function is worthy of consideration. The aim of the study was to assess ghrelin and obestatin immunocytochemical expression in nodular goiter (NG), papillary cancer (PTC) and medullary cancer (MTC).

Material and methods. Analyzed samples included 9 cases of NG, 8 cases of PTC and 11 cases of MTC. The analysis of ghrelin and obestatin expression was performed by use of the immunohistochemical (IHC) EnVision system and evaluated with filter HSV software (quantitative morphometric analysis).

Results. Quantitative ghrelin expression in MTC cells was higher than in NG (p = 0.013) and correlated negatively with the size of the tumor (r= –0.829, p < 0.05). We did not observe any differences in ghrelin expression neither between MTC and PTC nor between NG and PTC. Obestatin immunoexpression pattern in all analyzed specimens was irregular and poorly accented. The strongest immunoreactivity for obestatin was demonstrated in NG. In MTC obestatin expression was significantly weaker than in NG and PTC (p < 0.05 in both cases). In NG the intensity of obestatin immunostaining was significantly higher than that of ghrelin (p = 0.03). Conversely, ghrelin expression in MTC was definitely more evident than obestatin immunoreactivity (p < 0.01). There was no statistically significant difference between ghrelin and obestatin expression in PTC. No correlations were detected between reciprocal tissue expressions of ghrelin and obestatin in the analyzed specimens of NG, PTC or MTC.

Conclusions. The differences between ghrelin expression in NG and MTC suggest that ghrelin may be involved in thyroid cell proliferation. The differences between ghrelin and obestatin immunoreactivity in benign and malignant thyroid tumors could support the theory of alternative transcription of the preproghrelin gene and independent production of ghrelin and obestatin.

Get Citation

Keywords

ghrelin; obestatin; thyroid; nodular goiter; papillary thyroid cancer; medullary thyroid cancer; IHC

About this article
Title

Ghrelin and obestatin in thyroid gland — immunohistochemical expression in nodular goiter, papillary and medullary cancer

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 53, No 1 (2015)

Pages

19-25

Published online

2015-04-14

DOI

10.5603/FHC.a2015.0004

Pubmed

25765090

Bibliographic record

Folia Histochem Cytobiol 2015;53(1):19-25.

Keywords

ghrelin
obestatin
thyroid
nodular goiter
papillary thyroid cancer
medullary thyroid cancer
IHC

Authors

Edyta Gurgul
Aldona Kasprzak
Agata Blaszczyk
Maciej Biczysko
Joanna Surdyk-Zasada
Agnieszka Seraszek-Jaros
Marek Ruchala

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