open access

Vol 52, No 4 (2014)
ORIGINAL PAPERS
Published online: 2014-01-26
Submitted: 2014-07-01
Accepted: 2014-10-23
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Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Karol Rycerz, Aleksandra Krawczyk, Jadwiga Jaworska-Adamu, Izabela Krawczyk-Marc
DOI: 10.5603/FHC.a2014.0030
·
Folia Histochem Cytobiol 2014;52(4):281-288.

open access

Vol 52, No 4 (2014)
ORIGINAL PAPERS
Published online: 2014-01-26
Submitted: 2014-07-01
Accepted: 2014-10-23

Abstract

Introduction. Calretinin (CR) is a protein, which is present in GABAergic neurons and belongs to the calcium-binding proteins family. It may reduce the excitotoxicity phenomenon through its Ca2+ buffering properties. This phenomenon is due to the increase of calcium ions levels caused by the excess of glutamate — the main excitatory neurotransmitter. The aim of the study was to investigate alterations of calretinin-immunoreactivity in neurons of hippocampal CA1 region and dentate gyrus with hilus in 10 day-old rats treated with monosodium glutamate (MSG).

Material and methods. Ten 7 day-old Wistar rats were used. The MSG-group consisted of 5 MSG-treated rats at a dose of 4 g/kg b.w. for 3 consecutive days and the second group consisted of 5 control animals. After euthanasia the brains containing hippocampus were dissected and embedded in paraffin blocks. The immunohi­stochemical peroxidase-antiperoxydase reaction was performed on tissue sections. The morphometric analyses of CR-immunopositive neurons: density, percentage ratio to the density of all cells and an assessment of digital immunostaining intensity were performed.

Results. The distribution of the CR-immunoreactive neurons in the hippocampus was irregular. In the MSG-group there were single cells, which were more intensely stained than in control animals. Some of cells contained processes of different length. The density of CR-immunopositive cells and their percentage ratio to the density of all cells did not change significantly after MSG treatment. However, there was a statistically significant increase in the staining intensity of CR-immunopositive cells.

Conclusions. The obtained results indicate that CR-positive cells in P7–P10 rats are only slightly affected by MSG in CA1 region and dentate gyrus with hilus of the hippocampus.

Abstract

Introduction. Calretinin (CR) is a protein, which is present in GABAergic neurons and belongs to the calcium-binding proteins family. It may reduce the excitotoxicity phenomenon through its Ca2+ buffering properties. This phenomenon is due to the increase of calcium ions levels caused by the excess of glutamate — the main excitatory neurotransmitter. The aim of the study was to investigate alterations of calretinin-immunoreactivity in neurons of hippocampal CA1 region and dentate gyrus with hilus in 10 day-old rats treated with monosodium glutamate (MSG).

Material and methods. Ten 7 day-old Wistar rats were used. The MSG-group consisted of 5 MSG-treated rats at a dose of 4 g/kg b.w. for 3 consecutive days and the second group consisted of 5 control animals. After euthanasia the brains containing hippocampus were dissected and embedded in paraffin blocks. The immunohi­stochemical peroxidase-antiperoxydase reaction was performed on tissue sections. The morphometric analyses of CR-immunopositive neurons: density, percentage ratio to the density of all cells and an assessment of digital immunostaining intensity were performed.

Results. The distribution of the CR-immunoreactive neurons in the hippocampus was irregular. In the MSG-group there were single cells, which were more intensely stained than in control animals. Some of cells contained processes of different length. The density of CR-immunopositive cells and their percentage ratio to the density of all cells did not change significantly after MSG treatment. However, there was a statistically significant increase in the staining intensity of CR-immunopositive cells.

Conclusions. The obtained results indicate that CR-positive cells in P7–P10 rats are only slightly affected by MSG in CA1 region and dentate gyrus with hilus of the hippocampus.

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Keywords

calretinin; neurons; hippocampus; rats; monosodium glutamate

About this article
Title

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 4 (2014)

Pages

281-288

Published online

2014-01-26

DOI

10.5603/FHC.a2014.0030

Bibliographic record

Folia Histochem Cytobiol 2014;52(4):281-288.

Keywords

calretinin
neurons
hippocampus
rats
monosodium glutamate

Authors

Karol Rycerz
Aleksandra Krawczyk
Jadwiga Jaworska-Adamu
Izabela Krawczyk-Marc

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