open access

Vol 52, No 3 (2014)
ORIGINAL PAPERS
Published online: 2014-10-10
Submitted: 2014-03-25
Accepted: 2014-09-09
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Cultivation of circulating tumor cells in esophageal cancer

Vladimir Bobek, Rafal Matkowski, Robert Gürlich, Krysztof Grabowski, Jolanta Szelachowska, Robert Lischke, Jan Schützner, Tomas Harustiak, Alexandr Pazdro, Adam Rzechonek, Katarina Kolostova
DOI: 10.5603/FHC.2014.0020
·
Folia Histochem Cytobiol 2014;52(3):171-177.

open access

Vol 52, No 3 (2014)
ORIGINAL PAPERS
Published online: 2014-10-10
Submitted: 2014-03-25
Accepted: 2014-09-09

Abstract

The presence of circulating tumor cells (CTCs) in patients with metastatic carcinoma is generally associated with poor clinical outcome. There have been many investigations showing a possible use of CTCs as minimally invasive predictive and prognostic biomarker in cancer medicine. In this report a size-based method (MetaCell®) for quick and easy enrichment and cultivation of CTCs is presented to enable possible CTCs use in esophageal cancer (EC) management. In total, 43 patients with diagnosed EC, 20 with adenocarcinoma (AdenoCa) and 23 with squamous cell carcinoma (SCC), were enrolled into the adaptive prospective-like study .All the patients were candidates for surgery. The CTCs were detected in 27 patients (62.8%), with a higher rate in adenocarcinoma (75%) than SCC (52%). Finally, there were 26 patients with resectable tumors exhibiting CTCs-positivity in 69.2% and 17 patients with non-resectable tumors with 41.7% CTCs-positivity. Interestingly, in the patients undergoing neoadjuvant therapy, the CTCs were detected at time of surgery in 55.5% (10/18). The overall size-based filtration approach enabled to isolate viable CTCs and evaluate to their cytomorphological features by means of vital fluorescent staining. The CTCs were cultured in vitro for further downstream applications including immunohistochemical analysis. This is the first report of the successful culturing of esophageal cancer CTCs. The detection of CTCs presence could help in the future to guide timing of surgical treatment in EC patients.

Abstract

The presence of circulating tumor cells (CTCs) in patients with metastatic carcinoma is generally associated with poor clinical outcome. There have been many investigations showing a possible use of CTCs as minimally invasive predictive and prognostic biomarker in cancer medicine. In this report a size-based method (MetaCell®) for quick and easy enrichment and cultivation of CTCs is presented to enable possible CTCs use in esophageal cancer (EC) management. In total, 43 patients with diagnosed EC, 20 with adenocarcinoma (AdenoCa) and 23 with squamous cell carcinoma (SCC), were enrolled into the adaptive prospective-like study .All the patients were candidates for surgery. The CTCs were detected in 27 patients (62.8%), with a higher rate in adenocarcinoma (75%) than SCC (52%). Finally, there were 26 patients with resectable tumors exhibiting CTCs-positivity in 69.2% and 17 patients with non-resectable tumors with 41.7% CTCs-positivity. Interestingly, in the patients undergoing neoadjuvant therapy, the CTCs were detected at time of surgery in 55.5% (10/18). The overall size-based filtration approach enabled to isolate viable CTCs and evaluate to their cytomorphological features by means of vital fluorescent staining. The CTCs were cultured in vitro for further downstream applications including immunohistochemical analysis. This is the first report of the successful culturing of esophageal cancer CTCs. The detection of CTCs presence could help in the future to guide timing of surgical treatment in EC patients.
Get Citation

Keywords

esophageal cancer; circulating tumor cells; MetaCell®; CTCs culture; gastrointestinal tumors

About this article
Title

Cultivation of circulating tumor cells in esophageal cancer

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 3 (2014)

Pages

171-177

Published online

2014-10-10

DOI

10.5603/FHC.2014.0020

Bibliographic record

Folia Histochem Cytobiol 2014;52(3):171-177.

Keywords

esophageal cancer
circulating tumor cells
MetaCell®
CTCs culture
gastrointestinal tumors

Authors

Vladimir Bobek
Rafal Matkowski
Robert Gürlich
Krysztof Grabowski
Jolanta Szelachowska
Robert Lischke
Jan Schützner
Tomas Harustiak
Alexandr Pazdro
Adam Rzechonek
Katarina Kolostova

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