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Vol 52, No 2 (2014)
Original paper
Submitted: 2014-03-04
Accepted: 2014-06-22
Published online: 2014-07-09
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The glycomic profile of invasive ductal carcinoma of the breast is altered in patients with hypoxic regions: implications for tumor behavior

Moacyr Jesus Barreto de Melo Rêgo, Antônio Felix da Silva Filho, Marina Ferraz Cordeiro, Petra Barros Santos, Eduardo Isidoro Carneiro Beltrão
DOI: 10.5603/FHC.2014.0017
·
Folia Histochem Cytobiol 2014;52(2):96-103.

open access

Vol 52, No 2 (2014)
ORIGINAL PAPERS
Submitted: 2014-03-04
Accepted: 2014-06-22
Published online: 2014-07-09

Abstract

Hypoxic areas in solid tumors are often associated with poor prognosis and resistance to chemotherapy. The aim of the study was to analyze the expression of galectin-1 (Gal-1), galectin-3 (Gal-3), sialic acid and b1–6 branched glycan structures in hypoxic environment of invasive ductal carcinoma (IDC) of the breast. We performed lectin histochemistry with phytohemag glutinin-L (L-PHA) and Sambucus nigra lectin (SNA); and immunohistochemistry for Gal-1, Gal-3, carbonic anhydrase IX, hypoxia-inducible factor, estrogen receptor (ER), progesterone receptor and human epidermal growth factor receptor type-2 for 86 IDC samples. Patients with markers positive for hypoxia were mostly ER-negative (p = 0.003) and presented with more nodal invasion than the non-hypoxic group (p = 0.0439). Concerning the glycobiological aspects, the hypoxic group expressed more of Gal-3 (p = 0.0021) and SNA ligands (p = 0.0498), however, there was no association between lectin- and galectin-staining and clinical and histopathological data. Our results suggest a change in the glycomic profile of patients within hypoxic regions of IDC. However, further studies are needed to evaluate the role of lectin- and galectin-ligands in tumor’s hypoxic environment, as well as their potential to be used as therapeutic targets.

Abstract

Hypoxic areas in solid tumors are often associated with poor prognosis and resistance to chemotherapy. The aim of the study was to analyze the expression of galectin-1 (Gal-1), galectin-3 (Gal-3), sialic acid and b1–6 branched glycan structures in hypoxic environment of invasive ductal carcinoma (IDC) of the breast. We performed lectin histochemistry with phytohemag glutinin-L (L-PHA) and Sambucus nigra lectin (SNA); and immunohistochemistry for Gal-1, Gal-3, carbonic anhydrase IX, hypoxia-inducible factor, estrogen receptor (ER), progesterone receptor and human epidermal growth factor receptor type-2 for 86 IDC samples. Patients with markers positive for hypoxia were mostly ER-negative (p = 0.003) and presented with more nodal invasion than the non-hypoxic group (p = 0.0439). Concerning the glycobiological aspects, the hypoxic group expressed more of Gal-3 (p = 0.0021) and SNA ligands (p = 0.0498), however, there was no association between lectin- and galectin-staining and clinical and histopathological data. Our results suggest a change in the glycomic profile of patients within hypoxic regions of IDC. However, further studies are needed to evaluate the role of lectin- and galectin-ligands in tumor’s hypoxic environment, as well as their potential to be used as therapeutic targets.

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Keywords

breast cancer; IDC; hypoxia; glycomic profile; galectins; lectin ligands; IHC; histochemistry

About this article
Title

The glycomic profile of invasive ductal carcinoma of the breast is altered in patients with hypoxic regions: implications for tumor behavior

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 2 (2014)

Article type

Original paper

Pages

96-103

Published online

2014-07-09

Page views

1701

Article views/downloads

1712

DOI

10.5603/FHC.2014.0017

Bibliographic record

Folia Histochem Cytobiol 2014;52(2):96-103.

Keywords

breast cancer
IDC
hypoxia
glycomic profile
galectins
lectin ligands
IHC
histochemistry

Authors

Moacyr Jesus Barreto de Melo Rêgo
Antônio Felix da Silva Filho
Marina Ferraz Cordeiro
Petra Barros Santos
Eduardo Isidoro Carneiro Beltrão

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