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Vol 52, No 2 (2014)
Original paper
Submitted: 2014-01-08
Accepted: 2014-05-15
Published online: 2014-07-09
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Effect of L-thyroxine treatment on peripheral blood dendritic cell subpopulations in patients with Hashimoto’s thyroiditis

Mariusz Stasiolek, Marek Dedecjus, Zbigniew Adamczewski, Przemyslaw Wiktor Sliwka, Jan Brzezinski, Andrzej Lewinski
DOI: 10.5603/FHC.2014.0013
·
Folia Histochem Cytobiol 2014;52(2):138-143.

open access

Vol 52, No 2 (2014)
ORIGINAL PAPERS
Submitted: 2014-01-08
Accepted: 2014-05-15
Published online: 2014-07-09

Abstract

Recent reports suggested dendritic cells (DCs) to be important players in the pathogenesis of autoimmune thyroid processes in humans. However, there are virtually no data addressing the influence of thyroid autoaggression-associated disturbances of thyrometabolic conditions on DCs biology. The aim of the study was to evaluate the influence of L-thyroxine supplementation on conventional and plasmacytoid peripheral blood DCs subtypes in patients with hypothyroidism due to Hashimoto’s thyroiditis (HT). Eighteen patients with newly diagnosed hypothyroidism due to HT were included into the study. All patients received L-thyroxine treatment with doses adjusted to reach euthyroidism. Peripheral blood DC subtypes structure and immunoregulatory phenotype were analyzed by flow cytometry in the same patient prospectively at two time points: (i) beforeand (ii) 3 months after beginning of L-thyroxine treatment (hypothyroidism vs. euthyroidism, respectively). Percentage of plasmacytoid DCs in peripheral blood mononuclear cells fraction was significantly decreased in the course of L-thyroxine treatment (0.27 ± 0.19 vs. 0.11 ± 0.08; p < 0.05), whereas we did not observe any changes in the number of conventional DCs. However, the phenotypic analysis showed a significant increase of conventional DCs expressing CD86 and CD91 (64.25 ± 21.6% vs. 86.3 ± 11%; p < 0.05 and 30.75 ± 11.66% vs. 44.5 ± 13.3%; p < 0.05; respectively) in euthyroid patients. Standard L-thyroxine supplementation in HT patients exerted significant immunoregulatory effects, associated with quantitative and phenotypic changes of peripheral blood DC subpopulations.

Abstract

Recent reports suggested dendritic cells (DCs) to be important players in the pathogenesis of autoimmune thyroid processes in humans. However, there are virtually no data addressing the influence of thyroid autoaggression-associated disturbances of thyrometabolic conditions on DCs biology. The aim of the study was to evaluate the influence of L-thyroxine supplementation on conventional and plasmacytoid peripheral blood DCs subtypes in patients with hypothyroidism due to Hashimoto’s thyroiditis (HT). Eighteen patients with newly diagnosed hypothyroidism due to HT were included into the study. All patients received L-thyroxine treatment with doses adjusted to reach euthyroidism. Peripheral blood DC subtypes structure and immunoregulatory phenotype were analyzed by flow cytometry in the same patient prospectively at two time points: (i) beforeand (ii) 3 months after beginning of L-thyroxine treatment (hypothyroidism vs. euthyroidism, respectively). Percentage of plasmacytoid DCs in peripheral blood mononuclear cells fraction was significantly decreased in the course of L-thyroxine treatment (0.27 ± 0.19 vs. 0.11 ± 0.08; p < 0.05), whereas we did not observe any changes in the number of conventional DCs. However, the phenotypic analysis showed a significant increase of conventional DCs expressing CD86 and CD91 (64.25 ± 21.6% vs. 86.3 ± 11%; p < 0.05 and 30.75 ± 11.66% vs. 44.5 ± 13.3%; p < 0.05; respectively) in euthyroid patients. Standard L-thyroxine supplementation in HT patients exerted significant immunoregulatory effects, associated with quantitative and phenotypic changes of peripheral blood DC subpopulations.

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Keywords

Hashimoto’s thyroiditis; L-thyroxine; hypothyroidism; autoimmunity; dendritic cells; CD86; CD91

About this article
Title

Effect of L-thyroxine treatment on peripheral blood dendritic cell subpopulations in patients with Hashimoto’s thyroiditis

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 52, No 2 (2014)

Article type

Original paper

Pages

138-143

Published online

2014-07-09

Page views

2019

Article views/downloads

2285

DOI

10.5603/FHC.2014.0013

Bibliographic record

Folia Histochem Cytobiol 2014;52(2):138-143.

Keywords

Hashimoto’s thyroiditis
L-thyroxine
hypothyroidism
autoimmunity
dendritic cells
CD86
CD91

Authors

Mariusz Stasiolek
Marek Dedecjus
Zbigniew Adamczewski
Przemyslaw Wiktor Sliwka
Jan Brzezinski
Andrzej Lewinski

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