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Vol 51, No 4 (2013)
Brief communication
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05
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Role of immunohistochemical and histochemical profiling in H&E-based diagnosis of scrotal leiomyosarcoma of dartos muscle

Andrzej Wincewicz, Piotr Lewitowicz, Urszula Sulkowska, Stanislaw Sulkowski, Agata Horecka-Lewitowicz, Dorota Koziel, Stanislaw Gluszek
DOI: 10.5603/FHC.2013.0046
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Folia Histochem Cytobiol 2013;51(4):339-342.

open access

Vol 51, No 4 (2013)
Short Communication
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05

Abstract

Abstract: Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dartos muscle. The tumor was diagnosed leiomyosarcoma G2 pT1b. Interestingly, the sarcomatous mass was focally strictly attached to convoluted, benign bundles of smooth muscles that were intermingled with tumor mass at peripheral, lateral and superior sides of the lesion. We have used immune- and histochemical methods to confirm histopathological findings based on H&E staining. As expected, in tumor cells smooth muscle actin and desmin were strongly immunopositive similarly as Masson trichrome staining, while S100 and CD34 antigens were immunonegative except for sustained positivity for CD34 in vessels. The auxiliary staining methods can provide additional information on the tumorigenesis of leiomyosarcoma. They can also serve to determine additional features of prognostic significance, since e.g. immunoreactivity of CD34 accurately maps vascular density of tumor and enables a careful assessment of vascular invasion in course of leiomyosarcoma as well.

Abstract

Abstract: Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dartos muscle. The tumor was diagnosed leiomyosarcoma G2 pT1b. Interestingly, the sarcomatous mass was focally strictly attached to convoluted, benign bundles of smooth muscles that were intermingled with tumor mass at peripheral, lateral and superior sides of the lesion. We have used immune- and histochemical methods to confirm histopathological findings based on H&E staining. As expected, in tumor cells smooth muscle actin and desmin were strongly immunopositive similarly as Masson trichrome staining, while S100 and CD34 antigens were immunonegative except for sustained positivity for CD34 in vessels. The auxiliary staining methods can provide additional information on the tumorigenesis of leiomyosarcoma. They can also serve to determine additional features of prognostic significance, since e.g. immunoreactivity of CD34 accurately maps vascular density of tumor and enables a careful assessment of vascular invasion in course of leiomyosarcoma as well.

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Keywords

leiomyosarcoma; dartos muscle; Masson trichrome staining; aSMA, desmin; CD34; origin of tumor

About this article
Title

Role of immunohistochemical and histochemical profiling in H&E-based diagnosis of scrotal leiomyosarcoma of dartos muscle

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 4 (2013)

Article type

Brief communication

Pages

339-342

Published online

2014-02-05

Page views

1248

Article views/downloads

1686

DOI

10.5603/FHC.2013.0046

Bibliographic record

Folia Histochem Cytobiol 2013;51(4):339-342.

Keywords

leiomyosarcoma
dartos muscle
Masson trichrome staining
aSMA
desmin
CD34
origin of tumor

Authors

Andrzej Wincewicz
Piotr Lewitowicz
Urszula Sulkowska
Stanislaw Sulkowski
Agata Horecka-Lewitowicz
Dorota Koziel
Stanislaw Gluszek

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