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Vol 51, No 4 (2013)
Original paper
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05
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The relationships between hypoxia-dependent markers: HIF-1alpha, EPO and EPOR in colorectal cancer

Marek Baltaziak, Andrzej Wincewicz, Luiza Kanczuga-Koda, Joanna M. Lotowska, Mariusz Koda, Urszula Sulkowska, Marcin Baltaziak, Monika Podbielski, Maria E. Sobaniec-Lotowska, Stanislaw Sulkowski
DOI: 10.5603/FHC.2013.0043
·
Folia Histochem Cytobiol 2013;51(4):320-325.

open access

Vol 51, No 4 (2013)
ORIGINAL PAPERS
Submitted: 2014-02-05
Accepted: 2014-02-05
Published online: 2014-02-05

Abstract

Abstract: Hypoxia triggers production of several cytoprotective proteins. Hypoxia-inducible factor 1alpha (HIF-1α) is a powerful stimulator of transcription of many genes, including erythropoietin (EPO) in hypoxia-affected cells. Recent data have also implicated signaling by EPO receptor (EPOR) as a new factor influencing tumor progression. The aim of the study was to detect by immunohistochemistry the presence of HIF-1α, EPO and EPOR in colorectal cancer (CRC) in reference to clinicopathological variables. We found the presence of the studied proteins in specimens of all 125 CRC patients which is suggestive of the occurrence of hypoxia in colorectal cancer tissues. The expression of HIF-1α correlated significantly with the presence of EPO and EPOR in all samples (P < 0.001, r = 0.549 and P < 0.001, r = 0.536, respectively). Significant correlations (from P < 0.024 to P < 0.001) were found in the analyses of CRC subgroups such as histopathological type tumor, tumor grade, tumor stage and patients with lymph nodes metastases. The same high significant correlations (P < 0.001) were observed in group of sex, age and tumor location. However, the values of the correlation coefficients (r) which usually ranged from 0.5 to 0.6 suggest the existence of independent or concurrent mechanism stimulating generation of these proteins in colorectal cancer.

Abstract

Abstract: Hypoxia triggers production of several cytoprotective proteins. Hypoxia-inducible factor 1alpha (HIF-1α) is a powerful stimulator of transcription of many genes, including erythropoietin (EPO) in hypoxia-affected cells. Recent data have also implicated signaling by EPO receptor (EPOR) as a new factor influencing tumor progression. The aim of the study was to detect by immunohistochemistry the presence of HIF-1α, EPO and EPOR in colorectal cancer (CRC) in reference to clinicopathological variables. We found the presence of the studied proteins in specimens of all 125 CRC patients which is suggestive of the occurrence of hypoxia in colorectal cancer tissues. The expression of HIF-1α correlated significantly with the presence of EPO and EPOR in all samples (P < 0.001, r = 0.549 and P < 0.001, r = 0.536, respectively). Significant correlations (from P < 0.024 to P < 0.001) were found in the analyses of CRC subgroups such as histopathological type tumor, tumor grade, tumor stage and patients with lymph nodes metastases. The same high significant correlations (P < 0.001) were observed in group of sex, age and tumor location. However, the values of the correlation coefficients (r) which usually ranged from 0.5 to 0.6 suggest the existence of independent or concurrent mechanism stimulating generation of these proteins in colorectal cancer.

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Keywords

HIF-1alpha; EPO; erythropoietin receptor; correlation; colorectal cancer; immunohistochemistry

About this article
Title

The relationships between hypoxia-dependent markers: HIF-1alpha, EPO and EPOR in colorectal cancer

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 4 (2013)

Article type

Original paper

Pages

320-325

Published online

2014-02-05

Page views

2261

Article views/downloads

2279

DOI

10.5603/FHC.2013.0043

Bibliographic record

Folia Histochem Cytobiol 2013;51(4):320-325.

Keywords

HIF-1alpha
EPO
erythropoietin receptor
correlation
colorectal cancer
immunohistochemistry

Authors

Marek Baltaziak
Andrzej Wincewicz
Luiza Kanczuga-Koda
Joanna M. Lotowska
Mariusz Koda
Urszula Sulkowska
Marcin Baltaziak
Monika Podbielski
Maria E. Sobaniec-Lotowska
Stanislaw Sulkowski

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