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Kainic acid induces expression of caveolin-1 in activated microglia in rat brain
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Abstract
Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30)
Abstract
Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30)
Keywords
caveolin 1 expression; brain; microglia; kainic acid; seizures; rat; immunohistochemistry
About this articleTitle
Kainic acid induces expression of caveolin-1 in activated microglia in rat brain
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
25-30
Published online
2013-04-24
Page views
1723
Article views/downloads
2289
DOI
10.5603/FHC.2013.004
Bibliographic record
Folia Histochem Cytobiol 2013;51(1):25-30.
Keywords
caveolin 1 expression
brain
microglia
kainic acid
seizures
rat
immunohistochemistry
Authors
Shigeko Takeuchi
Wakoto Matsuda
Ikuo Tooyama
Osamu Yasuhara
