Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer
Abstract
Malignant pleural effusions (MPE) are a common clinical problem in patients with neoplastic disease. Pleural fluid cytology is the simplest definitive method for obtaining a diagnosis of MPE. We describe a method that may increase the cancer cell detection rate using immunomagnetic separation in MPE. In comparison to standard MPE cytodiagnostic methods, we report a more streamlined method of isolation living cells that are able to proliferate. These captured cells can then be used for additional downstream analysis e.g. chemosensitivity testing. Several case studies of MPE diagnostics using immunomagnetic separation are presented in the following report. The immunomagnetic separation of cancer cells from MPE could be used for more accurate staging of patients with routine effusions.
Abstract
Malignant pleural effusions (MPE) are a common clinical problem in patients with neoplastic disease. Pleural fluid cytology is the simplest definitive method for obtaining a diagnosis of MPE. We describe a method that may increase the cancer cell detection rate using immunomagnetic separation in MPE. In comparison to standard MPE cytodiagnostic methods, we report a more streamlined method of isolation living cells that are able to proliferate. These captured cells can then be used for additional downstream analysis e.g. chemosensitivity testing. Several case studies of MPE diagnostics using immunomagnetic separation are presented in the following report. The immunomagnetic separation of cancer cells from MPE could be used for more accurate staging of patients with routine effusions.
Keywords
pleural effusion; cancer cell isolation; immunomagnetic beads; cell culture
Title
Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
201-205
Published online
2013-11-07
Page views
1855
Article views/downloads
1252
DOI
10.5603/FHC.2013.0028
Bibliographic record
Folia Histochem Cytobiol 2013;51(3):201-205.
Keywords
pleural effusion
cancer cell isolation
immunomagnetic beads
cell culture
Authors
Grzegorz Kacprzak
Katarina Kolostova
Jerzy Kolodziej
Ireneusz Pawlak
Adam Rzechonek
Vladimir Bobek