open access
Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion
open access
Abstract
a serious otological problem in children. Cytokines, potent inflammatory mediators, play important role in the
initiation of immunological response in otitis media. Adenoids excised due to hypertrophy with or without chronic
otitis media with effusion were used to isolate mononuclear cells. Secretion of cytokines by non-stimulated and
PHA-stimulated cells was determined by specific ELISAs. We found a significant increase in the production of
IL-5 and TNF-a secreted by adenoidal cells of children with otitis media with effusion compared to group with
hypertrophic adenoids. No differences were found in the secretion of IL-8, IL-6, and IL-10 between these two
groups of patients. Our results suggest a difference between the immunological responses in the course of hypertrophic
adenoids with otitis media as compared to hypertrophic adenoids.
Abstract
a serious otological problem in children. Cytokines, potent inflammatory mediators, play important role in the
initiation of immunological response in otitis media. Adenoids excised due to hypertrophy with or without chronic
otitis media with effusion were used to isolate mononuclear cells. Secretion of cytokines by non-stimulated and
PHA-stimulated cells was determined by specific ELISAs. We found a significant increase in the production of
IL-5 and TNF-a secreted by adenoidal cells of children with otitis media with effusion compared to group with
hypertrophic adenoids. No differences were found in the secretion of IL-8, IL-6, and IL-10 between these two
groups of patients. Our results suggest a difference between the immunological responses in the course of hypertrophic
adenoids with otitis media as compared to hypertrophic adenoids.
Title
Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
586-589
Published online
2012-12-23
Page views
2141
Article views/downloads
2380
DOI
10.5603/FHC.2012.0082
Bibliographic record
Folia Histochem Cytobiol 2012;50(4):586-589.
Authors
Beata Zelazowska-Rutkowska
Elzbieta Ilendo
Bozena Skotnicka
Jolanta Wysocka
Edwina Kasprzycka