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Vol 50, No 2 (2012)
Original paper
Submitted: 2012-01-19
Accepted: 2012-01-19
Published online: 2012-07-05
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Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

Saied Karbalay-Doust, Ali Noorafshan
DOI: 10.5603/FHC.2012.0037
·
Folia Histochem Cytobiol 2012;50(2):275-279.

open access

Vol 50, No 2 (2012)
ORIGINAL PAPERS
Submitted: 2012-01-19
Accepted: 2012-01-19
Published online: 2012-07-05

Abstract

Changes in the number and size of oocytes can lead to fertilization problems. The present study aimed to evaluate the number, volume, and surface area of oocytes in healthy as well as nandrolone decanoate-treated (ND) mice using stereological methods. Five control mice received vehicle, and five ND-treated mice received ND. Using the ‘isotropic Cavalieri’ design’, the ovary was sectioned. The volume of the ovary (cortex and medulla) was estimated. The oocytes’ volume and surface area were estimated using the invariator. The number of the oocytes was estimated using an optical disector. The volumes of the ovary, cortex, and medulla decreased ~50% in the ND-treated mice. The mean number (coefficient of variation) of preantral, antral, and atretic oocytes in the control ovary were 1,690 (0.29), 2,100 (0.52), and 3,900 (0.2), respectively, which decreased ~54%, ~87%, and ~91%, respectively in the ND-treated animals. The mean volume (coefficient of variation) of the preantral, antral, and atretic oocytes were 86,000 (0.27), 110,000 (0.48), and 27,000 (0.33) μm3, respectively. The mean surface area (coefficient of variation) of the three types of oocytes were 9,000 (0.24), 9,900 (0.28), and 4,700 (0.21) μm2, respectively. These parameters remained unchanged in the ND-treated mice. ND induces reduction in the number of oocytes, but not in the volume or the surface area.

Abstract

Changes in the number and size of oocytes can lead to fertilization problems. The present study aimed to evaluate the number, volume, and surface area of oocytes in healthy as well as nandrolone decanoate-treated (ND) mice using stereological methods. Five control mice received vehicle, and five ND-treated mice received ND. Using the ‘isotropic Cavalieri’ design’, the ovary was sectioned. The volume of the ovary (cortex and medulla) was estimated. The oocytes’ volume and surface area were estimated using the invariator. The number of the oocytes was estimated using an optical disector. The volumes of the ovary, cortex, and medulla decreased ~50% in the ND-treated mice. The mean number (coefficient of variation) of preantral, antral, and atretic oocytes in the control ovary were 1,690 (0.29), 2,100 (0.52), and 3,900 (0.2), respectively, which decreased ~54%, ~87%, and ~91%, respectively in the ND-treated animals. The mean volume (coefficient of variation) of the preantral, antral, and atretic oocytes were 86,000 (0.27), 110,000 (0.48), and 27,000 (0.33) μm3, respectively. The mean surface area (coefficient of variation) of the three types of oocytes were 9,000 (0.24), 9,900 (0.28), and 4,700 (0.21) μm2, respectively. These parameters remained unchanged in the ND-treated mice. ND induces reduction in the number of oocytes, but not in the volume or the surface area.

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Keywords

Stereology; Ovary; Oocyte; Nandrolone decanoate; Mouse

About this article
Title

Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 2 (2012)

Article type

Original paper

Pages

275-279

Published online

2012-07-05

Page views

2460

Article views/downloads

3643

DOI

10.5603/FHC.2012.0037

Bibliographic record

Folia Histochem Cytobiol 2012;50(2):275-279.

Keywords

Stereology
Ovary
Oocyte
Nandrolone decanoate
Mouse

Authors

Saied Karbalay-Doust
Ali Noorafshan

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