open access

Vol 50, No 2 (2012)
ORIGINAL PAPERS
Submitted: 2012-07-04
Accepted: 2012-07-04
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TLR4 ligation induces expression of APRIL molecule in human neutrophils — a preliminary study

Ewa Jabłońska, Natalia Wawrusiewicz-Kurylonek, Marzena Garley, Adam Krętowski
DOI: 10.5603/FHC.2012.0029
·
Folia Histochem Cytobiol 2012;50(2):196-202.

open access

Vol 50, No 2 (2012)
ORIGINAL PAPERS
Submitted: 2012-07-04
Accepted: 2012-07-04

Abstract

In the present study we investigate the consequences of TLR4 activation by LPS for the synthesis of a proliferation-inducing ligand (APRIL) by human neutrophils (PMNs), and the possible role of the ERK1/2 kinases signaling pathway. In order to make a comparison, the same examinations were carried out on autologous peripheral blood mononuclear cells (PBMCs). The levels of mRNA for APRIL and TLR4 were measured using the real-time PCR method. Western blot analysis was used to assay the expressions of APRIL and ERK1/2 in cell lysates. We discovered an increased expression of APRIL accompanying the increased expression of TLR4 in the LPS-stimulated PMNs and PBMCs. Furthermore, stimulation with LPS triggered similar changes in phospho-ERK1/2 proteins expression in those cells. The present study suggests that LPS plays a role in TLR4-ligation in APRIL induction through ERK1/2 pathway activation in human neutrophils and mononuclear cells of peripheral blood. The association between TLR4 activation and APRIL expression in examined leukocytes might have important implications for the  mmune response of the host exposed to TLR4 ligands such as LPS.

Abstract

In the present study we investigate the consequences of TLR4 activation by LPS for the synthesis of a proliferation-inducing ligand (APRIL) by human neutrophils (PMNs), and the possible role of the ERK1/2 kinases signaling pathway. In order to make a comparison, the same examinations were carried out on autologous peripheral blood mononuclear cells (PBMCs). The levels of mRNA for APRIL and TLR4 were measured using the real-time PCR method. Western blot analysis was used to assay the expressions of APRIL and ERK1/2 in cell lysates. We discovered an increased expression of APRIL accompanying the increased expression of TLR4 in the LPS-stimulated PMNs and PBMCs. Furthermore, stimulation with LPS triggered similar changes in phospho-ERK1/2 proteins expression in those cells. The present study suggests that LPS plays a role in TLR4-ligation in APRIL induction through ERK1/2 pathway activation in human neutrophils and mononuclear cells of peripheral blood. The association between TLR4 activation and APRIL expression in examined leukocytes might have important implications for the  mmune response of the host exposed to TLR4 ligands such as LPS.
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Keywords

neutrophils; mononuclear cells; proliferation-inducing ligand; toll-like receptor 4; extracellular signal-regulated kinases 1/2

About this article
Title

TLR4 ligation induces expression of APRIL molecule in human neutrophils — a preliminary study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 2 (2012)

Pages

196-202

DOI

10.5603/FHC.2012.0029

Bibliographic record

Folia Histochem Cytobiol 2012;50(2):196-202.

Keywords

neutrophils
mononuclear cells
proliferation-inducing ligand
toll-like receptor 4
extracellular signal-regulated kinases 1/2

Authors

Ewa Jabłońska
Natalia Wawrusiewicz-Kurylonek
Marzena Garley
Adam Krętowski

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