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Vol 50, No 1 (2012)
Original paper
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25
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Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Cα in chondrocytes

Qingzhi Chen, Bing Zhang, Tingting Yi, Chun Xia
DOI: 10.5603/FHC.2012.0020
·
Folia Histochem Cytobiol 2012;50(1):137-143.

open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25

Abstract

Protein kinase B (Akt) and protein kinase Cα (PKCα) play important roles in the regulation of cell apoptosis. The aim of this study was to investigate the expression of Akt and PKCa in chondrocytes of human knee osteoarthritic (OA) cartilage, further evaluating their role in chondrocyte apoptosis during OA progression. Human knee OA cartilages were obtained from 38 patients undergoing knee arthroplasty, which is the medium-late stage of OA. Healthy knee cartilages were obtained from 11 amputees. The samples taken from the condyle of femur were collected routinely for morphological, immunohistochemical and Western blot detection, respectively. Light microscopy and laser-scanning confocal microscopy were used for morphological observation. The optical density with computer image analysis evaluated the intensity of immunohistochemical reaction of Akt and PKCα in OA cartilage. Western blot detected the protein expression levels. The results indicated that Akt and PKCa were involved in OA progression, along with the increase of cell apoptosis. In OA cartilage, Akt decreased (p < 0.05) and PKCα increased (p < 0.05). There was a negative correlation and interaction between Akt and PKCα (r = –0.8). These results demonstrated that both Akt and PKCα are related to increased chondrocyte apoptosis in human OA cartilage. The correlation between human OA progression, the role of Akt and PKCα, and chondrocyte apoptosis allows for new therapeutic strategies to be considered.

Abstract

Protein kinase B (Akt) and protein kinase Cα (PKCα) play important roles in the regulation of cell apoptosis. The aim of this study was to investigate the expression of Akt and PKCa in chondrocytes of human knee osteoarthritic (OA) cartilage, further evaluating their role in chondrocyte apoptosis during OA progression. Human knee OA cartilages were obtained from 38 patients undergoing knee arthroplasty, which is the medium-late stage of OA. Healthy knee cartilages were obtained from 11 amputees. The samples taken from the condyle of femur were collected routinely for morphological, immunohistochemical and Western blot detection, respectively. Light microscopy and laser-scanning confocal microscopy were used for morphological observation. The optical density with computer image analysis evaluated the intensity of immunohistochemical reaction of Akt and PKCα in OA cartilage. Western blot detected the protein expression levels. The results indicated that Akt and PKCa were involved in OA progression, along with the increase of cell apoptosis. In OA cartilage, Akt decreased (p < 0.05) and PKCα increased (p < 0.05). There was a negative correlation and interaction between Akt and PKCα (r = –0.8). These results demonstrated that both Akt and PKCα are related to increased chondrocyte apoptosis in human OA cartilage. The correlation between human OA progression, the role of Akt and PKCα, and chondrocyte apoptosis allows for new therapeutic strategies to be considered.

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Keywords

Akt; PKCalpha; correlation; chondrocytes; human OA cartilage

About this article
Title

Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Cα in chondrocytes

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 1 (2012)

Article type

Original paper

Pages

137-143

Published online

2012-04-25

Page views

1892

Article views/downloads

2540

DOI

10.5603/FHC.2012.0020

Bibliographic record

Folia Histochem Cytobiol 2012;50(1):137-143.

Keywords

Akt
PKCalpha
correlation
chondrocytes
human OA cartilage

Authors

Qingzhi Chen
Bing Zhang
Tingting Yi
Chun Xia

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