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Vol 50, No 1 (2012)
Original paper
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25
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Expression of cyclin B1 after induction of senescence and cell death in non-small cell lung carcinoma A549 cells

Agnieszka Żuryń, Maciej Gagat, Aleksandra Antonina Grzanka, Lidia Gackowska, Alina Grzanka
DOI: 10.5603/FHC.2012.0008
·
Folia Histochem Cytobiol 2012;50(1):58-67.

open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25

Abstract

The purpose of this study was to evaluate the level of mitotic cyclin B1 in the context of senescence and cell death in A549 non-small cell lung carcinoma cells. This was performed through analysis of the cell cycle, the percentage of SA-β-galactosidase-positive, as well as TUNEL-positive cells. Morphological alterations were studied using a transmission electron microscope. Changes in  the intracellular level and the presence of cyclin B1 in the nucleus and cytoplasm areas were detected by flow cytometry and confocal fluorescence microscopy, respectively. In the cells exposed to various concentrations of doxorubicin, different kinds of cell death and senescent phenotype were observed. Alterations in the cell cycle and increased polyploidy may be indicative of mitotic catastrophe execution. Changes in cyclin B1 may also be strictly related to its different regulation at mitotic catastrophe and senescence programs.

Abstract

The purpose of this study was to evaluate the level of mitotic cyclin B1 in the context of senescence and cell death in A549 non-small cell lung carcinoma cells. This was performed through analysis of the cell cycle, the percentage of SA-β-galactosidase-positive, as well as TUNEL-positive cells. Morphological alterations were studied using a transmission electron microscope. Changes in  the intracellular level and the presence of cyclin B1 in the nucleus and cytoplasm areas were detected by flow cytometry and confocal fluorescence microscopy, respectively. In the cells exposed to various concentrations of doxorubicin, different kinds of cell death and senescent phenotype were observed. Alterations in the cell cycle and increased polyploidy may be indicative of mitotic catastrophe execution. Changes in cyclin B1 may also be strictly related to its different regulation at mitotic catastrophe and senescence programs.

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Keywords

cyclin B1; A549; senescence; cell death

About this article
Title

Expression of cyclin B1 after induction of senescence and cell death in non-small cell lung carcinoma A549 cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 1 (2012)

Article type

Original paper

Pages

58-67

Published online

2012-04-25

Page views

1960

Article views/downloads

2350

DOI

10.5603/FHC.2012.0008

Bibliographic record

Folia Histochem Cytobiol 2012;50(1):58-67.

Keywords

cyclin B1
A549
senescence
cell death

Authors

Agnieszka Żuryń
Maciej Gagat
Aleksandra Antonina Grzanka
Lidia Gackowska
Alina Grzanka

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