Vol 49, No 4 (2011)
Original paper
Submitted: 2012-01-16
Published online: 2012-01-16
Prognostic value of the immunological phenomena and relationship with clinicopathological characteristics of the tumor — the expression of the early CD69+, CD71+and the late CD25+, CD26+, HLA/DR + activation markers on T CD4+ and CD8+ lymphocytes in squamous cell laryngeal carcinoma. Part II
Katarzyna Starska, Ewa Głowacka, Andrzej Kulig, Iwona Lewy-Trenda, Magdalena Bryś, Przemysław Lewkowicz
DOI: 10.5603/FHC.2011.0082
·
Folia Histochem Cytobiol 2011;49(4):593-603.
Vol 49, No 4 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-16
Published online: 2012-01-16
Abstract
One of the most important challenges in contemporary oncology is to find objective biomarkers of tumor
aggressiveness, which help to identify more invasive phenotypes of the carcinoma. The purpose of this study was to
investigate the relationships between the early and the late activation markers expression on T CD4+ and CD8+
cells subpopulations and certain clinicopathological characteristics of the neoplastic infiltration in order to determine
their role as biomarkers for tumor behavior in squamous cell laryngeal carcinoma. Analysis of the early
(CD69+, CD71+) and the late activation antigens (CD25+
high, CD26+, HLA/DR+) expression on T CD4+ and CD8+
lymphocytes by cytofluorymetry in 55 patients treated for squamous cell laryngeal carcinoma was performed. Clinicomorphological
analysis on the basis of TNM criteria and tumor front grading, which included tumor-related
features and adjacent stroma-related characteristics of the peripheral edge of infiltration was carried out. The
relationships between the activation markers expression and parameters of tumor aggressiveness were investigated.
Our work revealed statistically significant differences in the expression of the studied activation markers on
T cells with regard to certain clinicomorphological fetaures. The expressions of CD69+ and CD71+ antigens on
T CD3+CD4+ and CD3+CD8+ cells as well as CD4+HLA/DR+ markers were higher for pT3 and pT4 tumors, in comparison with pT2 carcinomas. Moreover, tumors with the smallest number of TFG points were characterized
by significantly lower values of the average expression of CD3+CD69+ and CD3+CD71+ as well as CD4+HLA/DR+
markers on T lymphocytes. In addition, more aggressive and deeply infiltrating laryngeal carcinomas were most
often characterized by significantly higher values of the average expression of CD69+ and CD71+ antigens on CD8+
as well as HLA/DR+ markers on CD4+. Our study confirmed the implication of the early and the late activation
antigens expression on CD4+ and CD8+ T lymphocytes in clinicomorphological parameters of the tumor, especially
TFG total score and depth of invasion, and their importance as indicators of the invasive phenotype of laryngeal
carcinoma. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 593–603)
Abstract
One of the most important challenges in contemporary oncology is to find objective biomarkers of tumor
aggressiveness, which help to identify more invasive phenotypes of the carcinoma. The purpose of this study was to
investigate the relationships between the early and the late activation markers expression on T CD4+ and CD8+
cells subpopulations and certain clinicopathological characteristics of the neoplastic infiltration in order to determine
their role as biomarkers for tumor behavior in squamous cell laryngeal carcinoma. Analysis of the early
(CD69+, CD71+) and the late activation antigens (CD25+
high, CD26+, HLA/DR+) expression on T CD4+ and CD8+
lymphocytes by cytofluorymetry in 55 patients treated for squamous cell laryngeal carcinoma was performed. Clinicomorphological
analysis on the basis of TNM criteria and tumor front grading, which included tumor-related
features and adjacent stroma-related characteristics of the peripheral edge of infiltration was carried out. The
relationships between the activation markers expression and parameters of tumor aggressiveness were investigated.
Our work revealed statistically significant differences in the expression of the studied activation markers on
T cells with regard to certain clinicomorphological fetaures. The expressions of CD69+ and CD71+ antigens on
T CD3+CD4+ and CD3+CD8+ cells as well as CD4+HLA/DR+ markers were higher for pT3 and pT4 tumors, in comparison with pT2 carcinomas. Moreover, tumors with the smallest number of TFG points were characterized
by significantly lower values of the average expression of CD3+CD69+ and CD3+CD71+ as well as CD4+HLA/DR+
markers on T lymphocytes. In addition, more aggressive and deeply infiltrating laryngeal carcinomas were most
often characterized by significantly higher values of the average expression of CD69+ and CD71+ antigens on CD8+
as well as HLA/DR+ markers on CD4+. Our study confirmed the implication of the early and the late activation
antigens expression on CD4+ and CD8+ T lymphocytes in clinicomorphological parameters of the tumor, especially
TFG total score and depth of invasion, and their importance as indicators of the invasive phenotype of laryngeal
carcinoma. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 593–603)
Keywords
laryngeal carcinoma; T cells activation markers; clinicomorphological features
Title
Prognostic value of the immunological phenomena and relationship with clinicopathological characteristics of the tumor — the expression of the early CD69+, CD71+and the late CD25+, CD26+, HLA/DR + activation markers on T CD4+ and CD8+ lymphocytes in squamous cell laryngeal carcinoma. Part II
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 4 (2011)
Article type
Original paper
Pages
593-603
Published online
2012-01-16
Page views
1763
Article views/downloads
2111
DOI
10.5603/FHC.2011.0082
Bibliographic record
Folia Histochem Cytobiol 2011;49(4):593-603.
Keywords
laryngeal carcinoma
T cells activation markers
clinicomorphological features
Authors
Katarzyna Starska
Ewa Głowacka
Andrzej Kulig
Iwona Lewy-Trenda
Magdalena Bryś
Przemysław Lewkowicz