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Vol 49, No 4 (2011)
Original paper
Submitted: 2012-01-16
Published online: 2012-01-16
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The effect of ursolic and oleanolic acids on human skin fibroblast cells

Magdalena Wójciak-Kosior, Roman Paduch, Anna Matysik-Woźniak, Piotr Niedziela, Helena Donica
DOI: 10.5603/FHC.2011.0050
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Folia Histochem Cytobiol 2011;49(4):664-669.

open access

Vol 49, No 4 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-16
Published online: 2012-01-16

Abstract

In this article, we look at how ursolic and oleanolic acids can be used for the purpose of quality control of natural products used in dermatocosmetology as well as of various other therapeutic preparations. Ursolic acid (UA) and oleanolic acid (OA) are pentacyclic triterpenes and they are constituents of many medicinal herbs. In this study, we analyzed the cytotoxic and anti-proliferative activity of OA and UA against normal human skin fibroblasts (HSF). Additionally, the scavenging activity of free radicals of both acids was analyzed. The sensitivity of cells to OA and UA activity was determined using a standard spectrophotometric (MTT) assay. The free radical scavenging activity of OA and UA was measured using the DPPH• test. The F-actin cytoskeletal proteins organization was analyzed using TRITC-phalloidine fluorescent staining. The cytotoxic activity of the analyzed acids was determined using Neutral Red (NR) uptake assay. Of the two isomeric compounds, UA showed a higher cytotoxic activity against HSF cells than did OA. Our investigations showed that OA, in view of its non-toxic nature, may be used as a supplementary factor for dermal preparations. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 664–669)

Abstract

In this article, we look at how ursolic and oleanolic acids can be used for the purpose of quality control of natural products used in dermatocosmetology as well as of various other therapeutic preparations. Ursolic acid (UA) and oleanolic acid (OA) are pentacyclic triterpenes and they are constituents of many medicinal herbs. In this study, we analyzed the cytotoxic and anti-proliferative activity of OA and UA against normal human skin fibroblasts (HSF). Additionally, the scavenging activity of free radicals of both acids was analyzed. The sensitivity of cells to OA and UA activity was determined using a standard spectrophotometric (MTT) assay. The free radical scavenging activity of OA and UA was measured using the DPPH• test. The F-actin cytoskeletal proteins organization was analyzed using TRITC-phalloidine fluorescent staining. The cytotoxic activity of the analyzed acids was determined using Neutral Red (NR) uptake assay. Of the two isomeric compounds, UA showed a higher cytotoxic activity against HSF cells than did OA. Our investigations showed that OA, in view of its non-toxic nature, may be used as a supplementary factor for dermal preparations. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 664–669)

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Keywords

oleanolic acid; ursolic acid; HSF cell

About this article
Title

The effect of ursolic and oleanolic acids on human skin fibroblast cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 4 (2011)

Article type

Original paper

Pages

664-669

Published online

2012-01-16

Page views

2989

Article views/downloads

4112

DOI

10.5603/FHC.2011.0050

Bibliographic record

Folia Histochem Cytobiol 2011;49(4):664-669.

Keywords

oleanolic acid
ursolic acid
HSF cell

Authors

Magdalena Wójciak-Kosior
Roman Paduch
Anna Matysik-Woźniak
Piotr Niedziela
Helena Donica

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