Vol 49, No 2 (2011)
Original paper
Submitted: 2011-12-19
Published online: 2011-07-11
The immunohistochemical demonstration of parafollicular cells and evaluation of calcium-phosphate balance in patients with thyroid hemiagenesis
Marek Ruchala, Ewelina Szczepanek, Patrycja Sujka-Kordowska, Maciej Zabel, Maciej Biczysko, Jerzy Sowinski
DOI: 10.5603/FHC.2011.0042
·
Folia Histochem Cytobiol 2011;49(2):299-305.
Vol 49, No 2 (2011)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-07-11
Abstract
Thyroid hemiagenesis (TH) is characterized by the congenital absence of one thyroid lobe. The aim of this
study was to evaluate the calcium-phosphate balance in TH. Twenty patients with TH and 20 controls with a bilobed
thyroid were studied. Serum concentrations of total calcium, parathormon and calcitonin were measured. Additionally,
the immunohistochemical expression of calcitonin, chromogranin A (chA), neuron-specific enolase (NSE) and
calcitonin gene-related peptide (CGRP) was evaluated in surgical specimens from patients with TH and controls.
There were no significant differences in biochemical parameters between TH and controls. Positive staining for
calcitonin was demonstrated in 3/8 thyroid sections from three patients with TH, but only in 2/33 sections from four
controls (p < 0.005). All sections from patients with TH positive for calcitonin also expressed chA, NSE and CGRP.
Two sections from controls positive for calcitonin presented an additionally positive reaction for chA, and one of them
also for NSE. None presented positive staining for CGRP. Of three TH sections, in one, hyperplasia of C cells of
medium grade, and in another hyperplasia of C cells of high grade, could be detected. In the controls, hyperplasia of
C cells of low and medium grade was observed. TH was associated with slightly enhanced C cells hyperplasia compared
to controls, which might indicate compensatory proliferation. However, the calcium-phosphate balance does
not seem to be significantly affected. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 299–305)
Abstract
Thyroid hemiagenesis (TH) is characterized by the congenital absence of one thyroid lobe. The aim of this
study was to evaluate the calcium-phosphate balance in TH. Twenty patients with TH and 20 controls with a bilobed
thyroid were studied. Serum concentrations of total calcium, parathormon and calcitonin were measured. Additionally,
the immunohistochemical expression of calcitonin, chromogranin A (chA), neuron-specific enolase (NSE) and
calcitonin gene-related peptide (CGRP) was evaluated in surgical specimens from patients with TH and controls.
There were no significant differences in biochemical parameters between TH and controls. Positive staining for
calcitonin was demonstrated in 3/8 thyroid sections from three patients with TH, but only in 2/33 sections from four
controls (p < 0.005). All sections from patients with TH positive for calcitonin also expressed chA, NSE and CGRP.
Two sections from controls positive for calcitonin presented an additionally positive reaction for chA, and one of them
also for NSE. None presented positive staining for CGRP. Of three TH sections, in one, hyperplasia of C cells of
medium grade, and in another hyperplasia of C cells of high grade, could be detected. In the controls, hyperplasia of
C cells of low and medium grade was observed. TH was associated with slightly enhanced C cells hyperplasia compared
to controls, which might indicate compensatory proliferation. However, the calcium-phosphate balance does
not seem to be significantly affected. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 299–305)
Keywords
immunohistochemistry; parafollicular cells; calcium-phosphate balance; thyroid hemiagenesis
Title
The immunohistochemical demonstration of parafollicular cells and evaluation of calcium-phosphate balance in patients with thyroid hemiagenesis
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 2 (2011)
Article type
Original paper
Pages
299-305
Published online
2011-07-11
Page views
1648
Article views/downloads
1890
DOI
10.5603/FHC.2011.0042
Bibliographic record
Folia Histochem Cytobiol 2011;49(2):299-305.
Keywords
immunohistochemistry
parafollicular cells
calcium-phosphate balance
thyroid hemiagenesis
Authors
Marek Ruchala
Ewelina Szczepanek
Patrycja Sujka-Kordowska
Maciej Zabel
Maciej Biczysko
Jerzy Sowinski