Vol 49, No 2 (2011)
Original paper
Submitted: 2011-12-19
Published online: 2011-07-11
Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice
Christiane Kunert-Keil, Tomasz Gredes, Silke Lucke, Sven Morgenstern, Agnieszka Mielczarek, Katarzyna Sporniak-Tutak, Tomasz Gedrange, Alexander Spassov
DOI: 10.5603/FHC.2011.0041
·
Folia Histochem Cytobiol 2011;49(2):291-298.
Vol 49, No 2 (2011)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-07-11
Abstract
Duchenne muscular dystrophy (DMD) and murine X-linked muscular dystrophy (mdx), its murine
model, are characterized by muscle damage and muscle weakness associated with inflammation and new vessel
formation. Caveolins, dystrophin-associated proteins, are involved in the pathogenesis of DMD, because increased
numbers of caveolae are found in DMD and mdx hindlimb muscles. Caveolae influence angiogenesis
due to their content of vascular endothelial growth factor (VEGF) receptors. Orofacial muscles in mdx mice
undergo muscle necrosis followed by muscle regeneration. To ascertain the role of caveolins and VEGF in the
pathogenesis of dystrophic masticatory muscles, we examined the expression of caveolin-1 (cav-1), caveolin-3
(cav-3) and VEGF in control and mdx mice. In mdx masticatory muscles, no changes in transcript and protein
levels of VEGF were found, whereas cav-1 and cav-3 expression was increased. Using immunohistochemistry,
a strong sarcolemmal staining of caveolin-3 in regenerated muscle fibers was found. Furthermore, immunohistochemistry
with the caveolin-1 antibody showed an increase in the amount of blood vessels in areas with regenerating
muscle fibers. Dystrophic masticatory muscles showed changes comparable to those of hindlimb muscles
in the expression of cav-1 and cav-3. The angiogenesis seems to be unaffected in the jaw muscles of mdx mice.
We speculate that the increased caveolin expression could cause extensive and efficient muscle regeneration.
(Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 291–298)
Abstract
Duchenne muscular dystrophy (DMD) and murine X-linked muscular dystrophy (mdx), its murine
model, are characterized by muscle damage and muscle weakness associated with inflammation and new vessel
formation. Caveolins, dystrophin-associated proteins, are involved in the pathogenesis of DMD, because increased
numbers of caveolae are found in DMD and mdx hindlimb muscles. Caveolae influence angiogenesis
due to their content of vascular endothelial growth factor (VEGF) receptors. Orofacial muscles in mdx mice
undergo muscle necrosis followed by muscle regeneration. To ascertain the role of caveolins and VEGF in the
pathogenesis of dystrophic masticatory muscles, we examined the expression of caveolin-1 (cav-1), caveolin-3
(cav-3) and VEGF in control and mdx mice. In mdx masticatory muscles, no changes in transcript and protein
levels of VEGF were found, whereas cav-1 and cav-3 expression was increased. Using immunohistochemistry,
a strong sarcolemmal staining of caveolin-3 in regenerated muscle fibers was found. Furthermore, immunohistochemistry
with the caveolin-1 antibody showed an increase in the amount of blood vessels in areas with regenerating
muscle fibers. Dystrophic masticatory muscles showed changes comparable to those of hindlimb muscles
in the expression of cav-1 and cav-3. The angiogenesis seems to be unaffected in the jaw muscles of mdx mice.
We speculate that the increased caveolin expression could cause extensive and efficient muscle regeneration.
(Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 291–298)
Keywords
mdx mice; muscular dystrophy; histopathology; masticatory muscles
Title
Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 2 (2011)
Article type
Original paper
Pages
291-298
Published online
2011-07-11
Page views
3198
Article views/downloads
2257
DOI
10.5603/FHC.2011.0041
Bibliographic record
Folia Histochem Cytobiol 2011;49(2):291-298.
Keywords
mdx mice
muscular dystrophy
histopathology
masticatory muscles
Authors
Christiane Kunert-Keil
Tomasz Gredes
Silke Lucke
Sven Morgenstern
Agnieszka Mielczarek
Katarzyna Sporniak-Tutak
Tomasz Gedrange
Alexander Spassov