Vol 49, No 2 (2011)
Original paper
Published online: 2011-07-11
Proinflammatory chemokine gene expression influences survival of patients with non-Hodgkin’s lymphoma
DOI: 10.5603/FHC.2011.0033
Folia Histochem Cytobiol 2011;49(2):240-247.
Abstract
The migration, survival and proliferation of cells is the basis for all physiologic and pathologic processes
in the human body. All these reactions are regulated by a complex chemokine network that guides lymphocytes
homing, chemotaxis, adhesion and interplay between immunologic system response cells. Chemokines
are also responsible for metastatic dissemination of cancers, including Hodgkin’s and non-Hodgkin’s lymphomas.
The purpose of this study was to determine chemokine gene expression (CXCL8, CXCL10, CCL2, CCL3,
CCL4 and CCL5) in lymphoma lymph nodes compared to their expression in reactive lymph nodes. We also
analyzed the influence of chemokine gene expression on the survival of lymphoma patients. Chemokine gene
expression was evaluated in 37 lymphoma lymph nodes and in 25 samples of reactive lymph nodes. Gene expression
of chemokines CXCL8, CXCL10, CCL2, CCL3, CCL4 and CCL5 was measured using the PCR method.
Statistical analysis was performed using CSS Statistica for Windows (version 7.0) software. Probability values <
< 0.05 were considered statistically significant and those between 0.05 and 0.1 as indicative of a trend. We found
lower CXCL8 and CXCL10 gene expression in lymphoma lymph nodes compared to reactive lymph nodes. In
the cases of CCL2 and CCL3, expression in lymphomas was higher than in reactive lymph nodes. Patients with
high expression of CCL2 and CXCL10 had shorter survival. (Folia Histochemica et Cytobiologica 2011; Vol. 49,
No. 2, pp. 240–247)
Keywords: lymphomachemokinesgene expression
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