Vol 49, No 2 (2011)
Original paper
Submitted: 2011-12-19
Published online: 2011-07-11
Investigations of hippocampal astrocytes in lipopolysaccharide-preconditioned rats in the pilocarpine model of epilepsy
Jadwiga Jaworska-Adamu, Mirosława Dmowska, Regina Cybulska, Aleksandra Krawczyk, Bożena Pawlikowska-Pawlęga
DOI: 10.5603/FHC.2011.0030
·
Folia Histochem Cytobiol 2011;49(2):219-224.
Vol 49, No 2 (2011)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-07-11
Abstract
The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine
model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult
male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitoneally
(ip) preconditioned with LPS at a dose of 0.5 mg/kg b.w. The control animals were administered (ip)
saline or LPS alone. The astrocytes in the control animals displayed characteristic stellate morphology. Examinations
of the astrocytes were performed on days one, three and 21 of the pilocarpine model of epilepsy (i.e. in
the acute, silent and chronic periods). The astrocytes of the CA1 and CA3 pyramidal layers of the hippocampus
were observed and analyzed at the structural and ultrastructural levels. It was demonstrated that on days one
and three, glial cells from both the nonpreconditioned and the LPS-preconditioned animals displayed similar
reactive changes, manifesting themselves as swelling of cell bodies, glial processes, and astrocytosis. Moreover,
reduction in cell organelles aggregated at one pole and the presence of vacuoles were observed. The most
pronounced astrogliosis and cell swelling occurred on day 21. We conclude that LPS has no effect on the morphology
of astrocytes in the pilocarpine model of epilepsy, unlike the results obtained by other authors in vitro.
(Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 219–224)
Abstract
The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine
model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult
male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitoneally
(ip) preconditioned with LPS at a dose of 0.5 mg/kg b.w. The control animals were administered (ip)
saline or LPS alone. The astrocytes in the control animals displayed characteristic stellate morphology. Examinations
of the astrocytes were performed on days one, three and 21 of the pilocarpine model of epilepsy (i.e. in
the acute, silent and chronic periods). The astrocytes of the CA1 and CA3 pyramidal layers of the hippocampus
were observed and analyzed at the structural and ultrastructural levels. It was demonstrated that on days one
and three, glial cells from both the nonpreconditioned and the LPS-preconditioned animals displayed similar
reactive changes, manifesting themselves as swelling of cell bodies, glial processes, and astrocytosis. Moreover,
reduction in cell organelles aggregated at one pole and the presence of vacuoles were observed. The most
pronounced astrogliosis and cell swelling occurred on day 21. We conclude that LPS has no effect on the morphology
of astrocytes in the pilocarpine model of epilepsy, unlike the results obtained by other authors in vitro.
(Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 219–224)
Keywords
epilepsy; astrocytes; lipopolysaccharide; hippocampus; rat
Title
Investigations of hippocampal astrocytes in lipopolysaccharide-preconditioned rats in the pilocarpine model of epilepsy
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 49, No 2 (2011)
Article type
Original paper
Pages
219-224
Published online
2011-07-11
Page views
1816
Article views/downloads
2054
DOI
10.5603/FHC.2011.0030
Bibliographic record
Folia Histochem Cytobiol 2011;49(2):219-224.
Keywords
epilepsy
astrocytes
lipopolysaccharide
hippocampus
rat
Authors
Jadwiga Jaworska-Adamu
Mirosława Dmowska
Regina Cybulska
Aleksandra Krawczyk
Bożena Pawlikowska-Pawlęga