Assessment of the pathway of apoptosis involving PAR-4, DAXX and ZIPK proteins in CLL patients and its relationship with the principal prognostic factors

Agnieszka Bojarska-Junak; Małgorzata Sieklucka; Iwona Hus; Ewa Wąsik-Szczepanek; Maria Luiza Kusz; Agata Surdacka; Sylwia Chocholska; Anna Dmoszyńska; Jacek Roliński

doi:10.5603/FHC.2011.0014

Vol 49, No 1 (2011)

Original paper

Submitted: 2011-12-19

Published online: 2011-04-19

View PDF

Download PDF file

Get Citation

Assessment of the pathway of apoptosis involving PAR-4, DAXX and ZIPK proteins in CLL patients and its relationship with the principal prognostic factors

Agnieszka Bojarska-Junak, Małgorzata Sieklucka, Iwona Hus, Ewa Wąsik-Szczepanek, Maria Luiza Kusz, Agata Surdacka, Sylwia Chocholska, Anna Dmoszyńska, Jacek Roliński

DOI: 10.5603/FHC.2011.0014

Folia Histochem Cytobiol 2011;49(1):98-103.

Vol 49, No 1 (2011)

ORIGINAL PAPERS

Submitted: 2011-12-19

Published online: 2011-04-19

Abstract

Par-4 (prostate apoptosis response-4) protein was originally found upregulated in prostate tumor cells undergoing apoptosis. Then it was further identified as a proapoptotic protein upregulated both in normal and leukemic lymphocytes. The aim of our study was to assess PAR-4 protein expression in the B cells of CLL patients and to examine its relationship with the expression of other proteins involved in the apoptosis process, such as DAXX, ZIPK and BCL-2. We found a positive relationship between PAR-4 and BCL-2 protein expression. Additionally, there was a positive correlation between PAR-4 and both DAXX and ZIPK protein expression. The results of our research were also analyzed in association with the principal CLL prognostic factors. There was a positive correlation between the expression of PAR-4 protein and the lactate dehydrogenase (LDH) serum concentration (p < 0.005). The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38⁺ cells (p < 0.05), as well as with CD38⁺/ZAP-70⁺ cells (p < 0.05). Moreover, we found a close relationship between LPL protein expression or LPL/ADAM29 MFI ratio and PAR-4 protein expression. Our results confirm the significance of apoptosis deregulation in CLL, and suggest a possible relationship between PAR-4 expression and the clinical course of the disease. This however requires further investigation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 98–103)

Abstract

Par-4 (prostate apoptosis response-4) protein was originally found upregulated in prostate tumor cells undergoing apoptosis. Then it was further identified as a proapoptotic protein upregulated both in normal and leukemic lymphocytes. The aim of our study was to assess PAR-4 protein expression in the B cells of CLL patients and to examine its relationship with the expression of other proteins involved in the apoptosis process, such as DAXX, ZIPK and BCL-2. We found a positive relationship between PAR-4 and BCL-2 protein expression. Additionally, there was a positive correlation between PAR-4 and both DAXX and ZIPK protein expression. The results of our research were also analyzed in association with the principal CLL prognostic factors. There was a positive correlation between the expression of PAR-4 protein and the lactate dehydrogenase (LDH) serum concentration (p < 0.005). The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38⁺ cells (p < 0.05), as well as with CD38⁺/ZAP-70⁺ cells (p < 0.05). Moreover, we found a close relationship between LPL protein expression or LPL/ADAM29 MFI ratio and PAR-4 protein expression. Our results confirm the significance of apoptosis deregulation in CLL, and suggest a possible relationship between PAR-4 expression and the clinical course of the disease. This however requires further investigation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 98–103)

Full Text:
View PDF Download PDF file

Get Citation

Keywords

PAR-4; DAXX; ZIPK; BCL-2; CD38; apoptosis; chronic lymphocytic leukemia

About this article

Title

Assessment of the pathway of apoptosis involving PAR-4, DAXX and ZIPK proteins in CLL patients and its relationship with the principal prognostic factors

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 1 (2011)

Article type

Original paper

Pages

98-103

Published online

2011-04-19

Page views

1923

Article views/downloads

1965

DOI

10.5603/FHC.2011.0014

Bibliographic record

Folia Histochem Cytobiol 2011;49(1):98-103.

Keywords

PAR-4
DAXX
ZIPK
BCL-2
CD38
apoptosis
chronic lymphocytic leukemia

Authors

Agnieszka Bojarska-Junak
Małgorzata Sieklucka
Iwona Hus
Ewa Wąsik-Szczepanek
Maria Luiza Kusz
Agata Surdacka
Sylwia Chocholska
Anna Dmoszyńska
Jacek Roliński