open access

Vol 59, No 3 (2021)
Original paper
Submitted: 2021-03-22
Accepted: 2021-09-19
Published online: 2021-09-20
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IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes

Wei Xiong1, Hua Chen2, Jiequan Lu3, Jie Ren3, Chen Nie1, Ruijuan Liang3, Feng Liu3, Baofeng Huang4, Yu Luo4
DOI: 10.5603/FHC.a2021.0019
·
Pubmed: 34542904
·
Folia Histochem Cytobiol 2021;59(3):195-202.
Affiliations
  1. Department of Emergency, Shenzhen People’s Hospital Longhua Branch, The Second Affiliated Hospital of Jinan Medical College, Shenzhen, China
  2. Department of Emergency, Shenzhen People’s Hospital, The Second Affiliated Hospital of Jinan Medical College, Shenzhen, China
  3. Department of Cardiology, Shenzhen People’s Hospital, The Second Affiliated Hospital of Jinan Medical College, Shenzhen, China
  4. Department of Gerontology, Shenzhen People’s Hospital, The Second Affiliated Hospital of Jinan Medical College, Shenzhen, China

open access

Vol 59, No 3 (2021)
ORIGINAL PAPERS
Submitted: 2021-03-22
Accepted: 2021-09-19
Published online: 2021-09-20

Abstract

Introduction. The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that the serum level of IL-39 significantly increased in patients with acute myocardial infarction. However, the role of IL-39 in cardiomyocyte apoptosis remains unclear.

Material and methods. In this study, the cultured mouse HL-1 cardiomyocytes were incubated with PBS, 0-100 ng/mL IL-39, 200 μM H2O2 or 20 μM Trolox.

Results. IL-39 promoted the production of intracellular reactive oxygen species (ROS) in a concentration dependent manner in HL-1 cardiomyocytes. IL-39 and H2O2 both significantly promoted the production of intracellular ROS, increased the level of intracellular CCL2, stimulated the apoptotic progress of cardiomyocytes, increased the mRNA and protein expression levels of Bax, caspase-3, and p-p38 MAPK, and decreased the mRNA and protein expression levels of Bcl-2. ROS production, CCL2 level, cardiomyocyte apoptosis, and expression of Bax, caspase-3, and p-p38 MAPK were significantly amplified by the administration of IL-39 combined with H2O2, and these processes were significantly alleviated by an antioxidant Trolox.

Conclusion. This study was novel in revealing that IL-39 promoted apoptosis by stimulating the phosphorylation of p38 MAPK in mouse HL-1 cardiomyocytes.

Abstract

Introduction. The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that the serum level of IL-39 significantly increased in patients with acute myocardial infarction. However, the role of IL-39 in cardiomyocyte apoptosis remains unclear.

Material and methods. In this study, the cultured mouse HL-1 cardiomyocytes were incubated with PBS, 0-100 ng/mL IL-39, 200 μM H2O2 or 20 μM Trolox.

Results. IL-39 promoted the production of intracellular reactive oxygen species (ROS) in a concentration dependent manner in HL-1 cardiomyocytes. IL-39 and H2O2 both significantly promoted the production of intracellular ROS, increased the level of intracellular CCL2, stimulated the apoptotic progress of cardiomyocytes, increased the mRNA and protein expression levels of Bax, caspase-3, and p-p38 MAPK, and decreased the mRNA and protein expression levels of Bcl-2. ROS production, CCL2 level, cardiomyocyte apoptosis, and expression of Bax, caspase-3, and p-p38 MAPK were significantly amplified by the administration of IL-39 combined with H2O2, and these processes were significantly alleviated by an antioxidant Trolox.

Conclusion. This study was novel in revealing that IL-39 promoted apoptosis by stimulating the phosphorylation of p38 MAPK in mouse HL-1 cardiomyocytes.

Get Citation

Keywords

apoptosis; HL-1 cardiomyocyte; interleukin-39; p38 MAPK; ROS

About this article
Title

IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 59, No 3 (2021)

Article type

Original paper

Pages

195-202

Published online

2021-09-20

DOI

10.5603/FHC.a2021.0019

Pubmed

34542904

Bibliographic record

Folia Histochem Cytobiol 2021;59(3):195-202.

Keywords

apoptosis
HL-1 cardiomyocyte
interleukin-39
p38 MAPK
ROS

Authors

Wei Xiong
Hua Chen
Jiequan Lu
Jie Ren
Chen Nie
Ruijuan Liang
Feng Liu
Baofeng Huang
Yu Luo

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