Vol 58, No 2 (2020)
Original paper
Published online: 2020-06-02
Immunocytochemical localization of tyrosine hydroxylase in the visual cortex of the microbat, Rhinolophus ferrumequinum
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Abstract
Introduction. In order to enhance our understanding of bat vision, we investigated tyrosine hydroxylase (TH)-immunoreactive (IR) fibers in the visual cortex of the microbat.
Material and methods. The study was conducted on 12 freshly-caught adult bats (Rhinolophus ferrumequinum,
both sexes, weighing 15–20 g). We used standard immunocytochemistry and confocal microscopy.
Results. TH-IR fibers were distributed throughout all layers of the visual cortex, with the highest density in layer I. Two types of TH-IR fibers were observed: small and large varicose fibers. TH-IR cells were not found in the microbat visual cortex. The microbat substantia nigra and ventral tegmental areas, previously identified sources of TH-IR fibers in the mammalian visual cortex, all contained strongly labeled TH-IR cells. The average diameters of TH-IR cells in the substantia nigra and the ventral tegmental areas were 14.39 ± 0.13 μm (mean ± SEM) and 11.85 ± 0.13 μm, respectively.
Conclusions. Our results suggest that the microbat has a well-constructed neurochemical organization of THIR fibers. This observation should provide fundamental insights into a better understanding of the nocturnal, echolocating bat visual system.
Keywords: visual cortextyrosine hydroxylasemicrobatimmunocytochemistry
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