open access

Vol 54, No 4 (2016)
Original paper
Submitted: 2016-07-06
Accepted: 2016-11-24
Published online: 2016-12-02
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Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Arturo Mangas, Javier Yajeya, Noelia Gonzalez, Marianne Husson, Michel Geffard, Rafael Coveñas
DOI: 10.5603/FHC.a2016.0024
·
Pubmed: 27966211
·
Folia Histochem Cytobiol 2016;54(4):186-192.

open access

Vol 54, No 4 (2016)
ORIGINAL PAPERS
Submitted: 2016-07-06
Accepted: 2016-11-24
Published online: 2016-12-02

Abstract

Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.

Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10–8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.

Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2–3 long (50–100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.

Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.

Abstract

Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.

Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10–8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.

Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2–3 long (50–100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.

Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.

Get Citation

Keywords

vitamin B5; pantothenic acid; new antibody; rat; brain; lateral septal nucleus; IHC

About this article
Title

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 54, No 4 (2016)

Article type

Original paper

Pages

186-192

Published online

2016-12-02

DOI

10.5603/FHC.a2016.0024

Pubmed

27966211

Bibliographic record

Folia Histochem Cytobiol 2016;54(4):186-192.

Keywords

vitamin B5
pantothenic acid
new antibody
rat
brain
lateral septal nucleus
IHC

Authors

Arturo Mangas
Javier Yajeya
Noelia Gonzalez
Marianne Husson
Michel Geffard
Rafael Coveñas

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