open access
Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody
open access
Abstract
Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.
Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10–8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.
Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2–3 long (50–100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.
Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.
Abstract
Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.
Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10–8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.
Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2–3 long (50–100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.
Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.
Keywords
vitamin B5; pantothenic acid; new antibody; rat; brain; lateral septal nucleus; IHC
About this articleTitle
Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
186-192
Published online
2016-12-02
Page views
1459
Article views/downloads
1819
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2016;54(4):186-192.
Keywords
vitamin B5
pantothenic acid
new antibody
rat
brain
lateral septal nucleus
IHC
Authors
Arturo Mangas
Javier Yajeya
Noelia Gonzalez
Marianne Husson
Michel Geffard
Rafael Coveñas
