open access

Vol 42, No 1 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-03-30
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Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression.

Krystyna Kozłowska, Mirosława Cichorek, Małgorzata Zarzeczna, Sławomir Wójcik
Folia Histochem Cytobiol 2004;42(1):29-34.

open access

Vol 42, No 1 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-03-30

Abstract

In the present work it was investigated if a spontaneous alteration of the native melanotic transplantable melanoma form into amelanotic form, connected with the tumor progression, is accompanied by changes of CD44 surface glycoprotein expression. We also tried to find out if there exists any correlation between changes in CD44 expression and IL-6, TNF-alpha, and IL-10 secretion. Cells of two hamster transplantable melanoma lines: melanotic and amelanotic were used. The levels of TNF-alpha, IL-6, IL-10 in supernatants were determined by the ELISA test. For the detection of CD44 expression by flow cytometry, isolated melanoma cells were stained with the rat anti-mouse CD44 monoclonal antibody. The stained cells were also examined using a fluorescence microscope and a confocal microscopy system. The obtained results indicate that a spontaneous alteration of the native melanotic form into amelanotic form and the associated tumor progression was accompanied by a decrease in CD44 glycoprotein expression on the cell surface and a decrease in IL-6, TNF-alpha and especially IL-10 secretion by amelanotic melanoma cells. Our observations suggest a relationship between CD44 expression and locally secreted cytokines in the course of transplantable melanoma progression.

Abstract

In the present work it was investigated if a spontaneous alteration of the native melanotic transplantable melanoma form into amelanotic form, connected with the tumor progression, is accompanied by changes of CD44 surface glycoprotein expression. We also tried to find out if there exists any correlation between changes in CD44 expression and IL-6, TNF-alpha, and IL-10 secretion. Cells of two hamster transplantable melanoma lines: melanotic and amelanotic were used. The levels of TNF-alpha, IL-6, IL-10 in supernatants were determined by the ELISA test. For the detection of CD44 expression by flow cytometry, isolated melanoma cells were stained with the rat anti-mouse CD44 monoclonal antibody. The stained cells were also examined using a fluorescence microscope and a confocal microscopy system. The obtained results indicate that a spontaneous alteration of the native melanotic form into amelanotic form and the associated tumor progression was accompanied by a decrease in CD44 glycoprotein expression on the cell surface and a decrease in IL-6, TNF-alpha and especially IL-10 secretion by amelanotic melanoma cells. Our observations suggest a relationship between CD44 expression and locally secreted cytokines in the course of transplantable melanoma progression.
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Keywords

CD44; Transplantable melanomas; Cytokines; IL-6 - IL-10 ; TNF-α

About this article
Title

Expression of CD44 on two lines of transplantable melanoma cells--relationship with cytokine secretion and tumor progression.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 1 (2004)

Article type

Original paper

Pages

29-34

Published online

2004-03-30

Bibliographic record

Folia Histochem Cytobiol 2004;42(1):29-34.

Keywords

CD44
Transplantable melanomas
Cytokines
IL-6 - IL-10
TNF-α

Authors

Krystyna Kozłowska
Mirosława Cichorek
Małgorzata Zarzeczna
Sławomir Wójcik

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