open access

Vol 42, No 2 (2004)
Original paper
Published online: 2004-07-16
Submitted: 2011-12-19
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Segmental distribution and morphometric features of primary sensory neurons projecting to the tibial periosteum in the rat.

Mariusz Gajda, Jan A Litwin, Dirk Adriaensen, Jean-Pierre Timmermans, Tadeusz Cichocki
Folia Histochem Cytobiol 2004;42(2):95-99.

open access

Vol 42, No 2 (2004)
ORIGINAL PAPERS
Published online: 2004-07-16
Submitted: 2011-12-19

Abstract

Previous reports have demonstrated very rich innervation pattern in the periosteum. Most of the periosteal fibers were found to be sensory in nature. The aim of this study was to identify the primary sensory neurons that innervate the tibial periosteum in the adult rat and to describe the morphometric features of their perikarya. To this end, an axonal fluorescent carbocyanine tracer, DiI, was injected into the periosteum on the medial surface of the tibia. The perikarya of the sensory fibers were traced back in the dorsal root ganglia (DRG) L1-L6 by means of fluorescent microscopy on cryosections. DiI-containing neurons were counted in each section and their segmental distribution was determined. Using PC-assisted image analysis system, the size and shape of the traced perikarya were analyzed. DiI-labeled sensory neurons innervating the periosteum of the tibia were located in the DRG ipsilateral to the injection site, with the highest distribution in L3 and L4 (57% and 23%, respectively). The majority of the traced neurons were of small size (area < 850 microm2), which is consistent with the size distribution of CGRP- and SP-containing cells, regarded as primary sensory neurons responsible for perception of pain and temperature. A small proportion of labeled cells had large perikarya and probably supplied corpuscular sense receptors observed in the periosteum. No differences were found in the shape distribution of neurons belonging to different size classes.

Abstract

Previous reports have demonstrated very rich innervation pattern in the periosteum. Most of the periosteal fibers were found to be sensory in nature. The aim of this study was to identify the primary sensory neurons that innervate the tibial periosteum in the adult rat and to describe the morphometric features of their perikarya. To this end, an axonal fluorescent carbocyanine tracer, DiI, was injected into the periosteum on the medial surface of the tibia. The perikarya of the sensory fibers were traced back in the dorsal root ganglia (DRG) L1-L6 by means of fluorescent microscopy on cryosections. DiI-containing neurons were counted in each section and their segmental distribution was determined. Using PC-assisted image analysis system, the size and shape of the traced perikarya were analyzed. DiI-labeled sensory neurons innervating the periosteum of the tibia were located in the DRG ipsilateral to the injection site, with the highest distribution in L3 and L4 (57% and 23%, respectively). The majority of the traced neurons were of small size (area < 850 microm2), which is consistent with the size distribution of CGRP- and SP-containing cells, regarded as primary sensory neurons responsible for perception of pain and temperature. A small proportion of labeled cells had large perikarya and probably supplied corpuscular sense receptors observed in the periosteum. No differences were found in the shape distribution of neurons belonging to different size classes.
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About this article
Title

Segmental distribution and morphometric features of primary sensory neurons projecting to the tibial periosteum in the rat.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 2 (2004)

Article type

Original paper

Pages

95-99

Published online

2004-07-16

Bibliographic record

Folia Histochem Cytobiol 2004;42(2):95-99.

Authors

Mariusz Gajda
Jan A Litwin
Dirk Adriaensen
Jean-Pierre Timmermans
Tadeusz Cichocki

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