open access

Vol 42, No 2 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-07-16
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In vivo accumulation of self-assembling dye Congo red in an area marked by specific immune complexes: possible relevance to chemotherapy.

Janina Rybarska, Barbara Piekarska, Barbara Stopa, Paweł Spólnik, Grzegorz Zemanek, Leszek Konieczny, Irena Roterman
Folia Histochem Cytobiol 2004;42(2):101-110.

open access

Vol 42, No 2 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-07-16

Abstract

Supramolecular micellar structures have been proposed as carriers in aim-oriented drug transportation to a target marked by specific immune complexes. In this study, the self-assembling dye Congo red was used as a model supramolecular carrier and its accumulation in the target was studied in vivo. The target was created in vivo as the local specific inflammation provoked by subcutaneous injection of antigen to the ear of a previously immunized rabbit. The color caused by accumulation of Congo red after its intravenous injection was registered by pictures of the ear with suitably filtered visible light shining through it to distinguish Congo red against the background color of hemoglobin. The results confirmed the expected accumulation and retention of Congo red in the inflammation area marked by deposits of specific immune complexes. The role of albumin and its possible interference with transportation of drugs through the blood by supramolecular carriers was also subjected to preliminary examination. The results revealed that albumin collaborates rather than interferes with drug transportation; this is another factor making the use of supramolecular carriers for aim-oriented chemotherapy highly promising.

Abstract

Supramolecular micellar structures have been proposed as carriers in aim-oriented drug transportation to a target marked by specific immune complexes. In this study, the self-assembling dye Congo red was used as a model supramolecular carrier and its accumulation in the target was studied in vivo. The target was created in vivo as the local specific inflammation provoked by subcutaneous injection of antigen to the ear of a previously immunized rabbit. The color caused by accumulation of Congo red after its intravenous injection was registered by pictures of the ear with suitably filtered visible light shining through it to distinguish Congo red against the background color of hemoglobin. The results confirmed the expected accumulation and retention of Congo red in the inflammation area marked by deposits of specific immune complexes. The role of albumin and its possible interference with transportation of drugs through the blood by supramolecular carriers was also subjected to preliminary examination. The results revealed that albumin collaborates rather than interferes with drug transportation; this is another factor making the use of supramolecular carriers for aim-oriented chemotherapy highly promising.
Get Citation
About this article
Title

In vivo accumulation of self-assembling dye Congo red in an area marked by specific immune complexes: possible relevance to chemotherapy.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 2 (2004)

Article type

Original paper

Pages

101-110

Published online

2004-07-16

Bibliographic record

Folia Histochem Cytobiol 2004;42(2):101-110.

Authors

Janina Rybarska
Barbara Piekarska
Barbara Stopa
Paweł Spólnik
Grzegorz Zemanek
Leszek Konieczny
Irena Roterman

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