Vol 42, No 2 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-07-16
Distribution of the DAZ gene transcripts in human testis.
A Szczerba, A Jankowska, M Andrusiewicz, M Karczewski, W Turkiewicz, J B Warchoł
Folia Histochem Cytobiol 2004;42(2):119-121.
Vol 42, No 2 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-07-16
Abstract
Involvement of variety of genes, especially located on Y chromosome, is critical for the regulation of spermatogenesis. In particular, fertility candidate genes such as deleted in azoospermia (DAZ) are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. The role of the DAZ gene is supported by its exclusive expression in the testis and by its deletion in about 10% of azoospermic and severely oligozoospermic patients. The distribution of DAZ transcripts in seminiferous epithelium of human testis is reported in the present study. The use of Adobe Photoshop and Scion Image softwares allowed for semi-quantitative analysis of in situ RT-PCR (ISRT-PCR) results. The intensity of ISRT-PCR product's fluorescence was different within individual seminiferous tubules. It was clearly shown by using the pseudocolour scale and transforming the intensity of the fluorescence into levels of greyscale images. The more intense fluorescence characterised single spermatogonia and those organized in small groups inside separate tubules. The most intense accumulation of DAZ mRNA was observed in spermatogonia.
Abstract
Involvement of variety of genes, especially located on Y chromosome, is critical for the regulation of spermatogenesis. In particular, fertility candidate genes such as deleted in azoospermia (DAZ) are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. The role of the DAZ gene is supported by its exclusive expression in the testis and by its deletion in about 10% of azoospermic and severely oligozoospermic patients. The distribution of DAZ transcripts in seminiferous epithelium of human testis is reported in the present study. The use of Adobe Photoshop and Scion Image softwares allowed for semi-quantitative analysis of in situ RT-PCR (ISRT-PCR) results. The intensity of ISRT-PCR product's fluorescence was different within individual seminiferous tubules. It was clearly shown by using the pseudocolour scale and transforming the intensity of the fluorescence into levels of greyscale images. The more intense fluorescence characterised single spermatogonia and those organized in small groups inside separate tubules. The most intense accumulation of DAZ mRNA was observed in spermatogonia.
Title
Distribution of the DAZ gene transcripts in human testis.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 42, No 2 (2004)
Article type
Original paper
Pages
119-121
Published online
2004-07-16
Page views
1313
Article views/downloads
929
Bibliographic record
Folia Histochem Cytobiol 2004;42(2):119-121.
Authors
A Szczerba
A Jankowska
M Andrusiewicz
M Karczewski
W Turkiewicz
J B Warchoł
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