Vol 42, No 3 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-10-21
Peritonitis-induced antitumor activity of peritoneal macrophages from uremic patients.
Bohdan Turyna, Aleksandra Jurek, Kamil Gotfryd, Agnieszka Siaśkiewicz, Piotr Kubit, Andrzej Klein
Folia Histochem Cytobiol 2004;42(3):147-153.
Vol 42, No 3 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-10-21
Abstract
The macrophages belong to the effector cells of both nonspecific and specific immune response. These cells generally express little cytotoxicity unless activated. The present work was intended to determine if peritoneal macrophages collected from patients on Continuous Ambulatory Peritoneal Dialysis (CAPD) during episodes of peritonitis were active against human tumor cell lines without further in vitro stimulation. We also compared macrophage antitumor potential with effectiveness of drugs used in cancer therapy (taxol and suramin). Conditioned medium (CM) of macrophages collected during inflammation-free periods did not exhibit cytostatic and cytotoxic activity against both tumor (A549 and HTB44) and non-transformed (BEAS-2B and CRL2190) cells. Exposure of tumor cells to CM of macrophages harvested during peritonitis resulted in significant suppression of proliferation, impairment of viability and induction of apoptosis, in contrast to non-transformed cells, which remained unaffected. The efficacy of CM of inflammatory macrophages as an antitumor agent appeared to be comparable to cytostatic and cytotoxic potency of taxol and suramin or, in the case of HTB44 cells, even higher. The results obtained suggest that activated human macrophages might represent a useful tool for cancer immunotherapy.
Abstract
The macrophages belong to the effector cells of both nonspecific and specific immune response. These cells generally express little cytotoxicity unless activated. The present work was intended to determine if peritoneal macrophages collected from patients on Continuous Ambulatory Peritoneal Dialysis (CAPD) during episodes of peritonitis were active against human tumor cell lines without further in vitro stimulation. We also compared macrophage antitumor potential with effectiveness of drugs used in cancer therapy (taxol and suramin). Conditioned medium (CM) of macrophages collected during inflammation-free periods did not exhibit cytostatic and cytotoxic activity against both tumor (A549 and HTB44) and non-transformed (BEAS-2B and CRL2190) cells. Exposure of tumor cells to CM of macrophages harvested during peritonitis resulted in significant suppression of proliferation, impairment of viability and induction of apoptosis, in contrast to non-transformed cells, which remained unaffected. The efficacy of CM of inflammatory macrophages as an antitumor agent appeared to be comparable to cytostatic and cytotoxic potency of taxol and suramin or, in the case of HTB44 cells, even higher. The results obtained suggest that activated human macrophages might represent a useful tool for cancer immunotherapy.
Title
Peritonitis-induced antitumor activity of peritoneal macrophages from uremic patients.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 42, No 3 (2004)
Article type
Original paper
Pages
147-153
Published online
2004-10-21
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1239
Article views/downloads
946
Bibliographic record
Folia Histochem Cytobiol 2004;42(3):147-153.
Authors
Bohdan Turyna
Aleksandra Jurek
Kamil Gotfryd
Agnieszka Siaśkiewicz
Piotr Kubit
Andrzej Klein
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