open access

Vol 42, No 3 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-10-21
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Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.

Albert Augustynowicz, Janusz Dziecioł, Małgorzata Barwijuk-Machała, Jacek Dadan, Zbigniew Puchalski, Stanisław Sulkowski
Folia Histochem Cytobiol 2004;42(3):165-168.

open access

Vol 42, No 3 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-10-21

Abstract

We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM.

Abstract

We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM.
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About this article
Title

Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 3 (2004)

Article type

Original paper

Pages

165-168

Published online

2004-10-21

Bibliographic record

Folia Histochem Cytobiol 2004;42(3):165-168.

Authors

Albert Augustynowicz
Janusz Dziecioł
Małgorzata Barwijuk-Machała
Jacek Dadan
Zbigniew Puchalski
Stanisław Sulkowski

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