Vol 42, No 3 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2004-10-21
Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.
Albert Augustynowicz, Janusz Dziecioł, Małgorzata Barwijuk-Machała, Jacek Dadan, Zbigniew Puchalski, Stanisław Sulkowski
Folia Histochem Cytobiol 2004;42(3):165-168.
Vol 42, No 3 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2004-10-21
Abstract
We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM.
Abstract
We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM.
Title
Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 42, No 3 (2004)
Article type
Original paper
Pages
165-168
Published online
2004-10-21
Page views
1136
Article views/downloads
1052
Bibliographic record
Folia Histochem Cytobiol 2004;42(3):165-168.
Authors
Albert Augustynowicz
Janusz Dziecioł
Małgorzata Barwijuk-Machała
Jacek Dadan
Zbigniew Puchalski
Stanisław Sulkowski
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