open access

Vol 42, No 4 (2004)
Original paper
Submitted: 2011-12-19
Published online: 2005-02-12
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mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis.

Krystyna Boczoń, Elzbieta Wandurska-Nowak, Andrzej Wierzbicki, Magdalena Frydrychowicz, Iwona Mozer-Lisewska, Jan Zeromski
Folia Histochem Cytobiol 2004;42(4):209-213.

open access

Vol 42, No 4 (2004)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2005-02-12

Abstract

The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis.

Abstract

The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis.
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About this article
Title

mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 4 (2004)

Article type

Original paper

Pages

209-213

Published online

2005-02-12

Bibliographic record

Folia Histochem Cytobiol 2004;42(4):209-213.

Authors

Krystyna Boczoń
Elzbieta Wandurska-Nowak
Andrzej Wierzbicki
Magdalena Frydrychowicz
Iwona Mozer-Lisewska
Jan Zeromski

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