open access

Vol 42, No 4 (2004)
ORIGINAL PAPERS
Published online: 2005-02-12
Submitted: 2011-12-19
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Immunoexpression of androgen receptors and aromatase in testes of patient with Klinefelter's syndrome.

Małgorzata Kotula-Balak, Leszek Bablok, Stanisław Fracki, Anna Jankowska, Barbara Bilińska
Folia Histochem Cytobiol 2004;42(4):215-220.

open access

Vol 42, No 4 (2004)
ORIGINAL PAPERS
Published online: 2005-02-12
Submitted: 2011-12-19

Abstract

Klinefelter's syndrome (47, XXY) is the most common chromosome aneuploidy in men and is usually characterized by underdeveloped testes and sterility. The aim of the present study was to detect cellular distribution of androgen receptors (AR) and aromatase in testes of patient with KS. The tissue sections were processed for morphological and immunohistochemical staining. Additionally, levels of FSH, LH, PRL, estradiol, and testosterone were measured in the plasma. Morphological analysis revealed a complete absence of spermatogenesis. No germ cells were present in seminiferous tubules. In some tubules, nests of apparently degenerating Sertoli cells were found. In the interstitium, Leydig cell hyperplasia was observed. Using immunohistochemistry, nuclear AR staining was detected in Sertoli cells and peritubular cells, whereas in Leydig cells the staining was exclusively cytoplasmic. The immunostaining of aromatase was detected in the cytoplasm of Sertoli cells and Leydig cells. Increased levels of gonadotropins and decreased level of testosterone concomitantly with the cytoplasmic localization of AR in Leydig cells might contribute to the impaired testicular function in patient with KS.

Abstract

Klinefelter's syndrome (47, XXY) is the most common chromosome aneuploidy in men and is usually characterized by underdeveloped testes and sterility. The aim of the present study was to detect cellular distribution of androgen receptors (AR) and aromatase in testes of patient with KS. The tissue sections were processed for morphological and immunohistochemical staining. Additionally, levels of FSH, LH, PRL, estradiol, and testosterone were measured in the plasma. Morphological analysis revealed a complete absence of spermatogenesis. No germ cells were present in seminiferous tubules. In some tubules, nests of apparently degenerating Sertoli cells were found. In the interstitium, Leydig cell hyperplasia was observed. Using immunohistochemistry, nuclear AR staining was detected in Sertoli cells and peritubular cells, whereas in Leydig cells the staining was exclusively cytoplasmic. The immunostaining of aromatase was detected in the cytoplasm of Sertoli cells and Leydig cells. Increased levels of gonadotropins and decreased level of testosterone concomitantly with the cytoplasmic localization of AR in Leydig cells might contribute to the impaired testicular function in patient with KS.
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About this article
Title

Immunoexpression of androgen receptors and aromatase in testes of patient with Klinefelter's syndrome.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 42, No 4 (2004)

Pages

215-220

Published online

2005-02-12

Bibliographic record

Folia Histochem Cytobiol 2004;42(4):215-220.

Authors

Małgorzata Kotula-Balak
Leszek Bablok
Stanisław Fracki
Anna Jankowska
Barbara Bilińska

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