open access

Vol 43, No 1 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-19
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Stereological analysis of mitochondria in embryos of Rana temporaria and Bufo bufo during cleavage

Marek Romek, Ewa Krzysztofowicz
Folia Histochem Cytobiol 2005;43(1):57-63.

open access

Vol 43, No 1 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-19

Abstract

Total numbers of mitochondria and their morphology have been quantitatively determined in mature oocytes and in cleaving embryos of two anuran species Rana temporaria and Bufo bufo using stereological methods. Surface densities of inner mitochondrial membranes for both studied species during cleavage ranged from 5.43 m2/cm3 to 7.53 m2/cm3, whereas volume densities of mitochondria did not exceed 1.65%. Since values of these parameters were low, thus embryos during cleavage may be considered as metabolically "silent". Transition of ultrastructural morphology of mitochondria towards that characterising actively respiring organelles occurs at stage 9 for R. temporaria and at stage 8 for B. bufo, correlated with blastula-gastrula and mid-blastula transition, respectively. The total numbers of mitochondria N(c) in mature oocytes are as high as 114.8 and 107.2 millions for R. temporaria and B. bufo, respectively, and during cleavage at late blastula stages they increase to 300 millions for both species under study. We suggest that an undefined mechanism might eliminate during cleavage those amphibian embryos which contain small number of mitochondria and low levels of nutrient substances.

Abstract

Total numbers of mitochondria and their morphology have been quantitatively determined in mature oocytes and in cleaving embryos of two anuran species Rana temporaria and Bufo bufo using stereological methods. Surface densities of inner mitochondrial membranes for both studied species during cleavage ranged from 5.43 m2/cm3 to 7.53 m2/cm3, whereas volume densities of mitochondria did not exceed 1.65%. Since values of these parameters were low, thus embryos during cleavage may be considered as metabolically "silent". Transition of ultrastructural morphology of mitochondria towards that characterising actively respiring organelles occurs at stage 9 for R. temporaria and at stage 8 for B. bufo, correlated with blastula-gastrula and mid-blastula transition, respectively. The total numbers of mitochondria N(c) in mature oocytes are as high as 114.8 and 107.2 millions for R. temporaria and B. bufo, respectively, and during cleavage at late blastula stages they increase to 300 millions for both species under study. We suggest that an undefined mechanism might eliminate during cleavage those amphibian embryos which contain small number of mitochondria and low levels of nutrient substances.
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Keywords

Mitochondria; Embryo; Cleavage; Rana; Bufo; Stereology

About this article
Title

Stereological analysis of mitochondria in embryos of Rana temporaria and Bufo bufo during cleavage

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 1 (2005)

Article type

Original paper

Pages

57-63

Published online

2011-08-19

Bibliographic record

Folia Histochem Cytobiol 2005;43(1):57-63.

Keywords

Mitochondria
Embryo
Cleavage
Rana
Bufo
Stereology

Authors

Marek Romek
Ewa Krzysztofowicz

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