Vol 43, No 1 (2005)
Original paper
Published online: 2011-08-19
Generation of dendritic cells from human peripheral blood monocytes - comparison of different culture media
Folia Histochem Cytobiol 2005;43(1):25-30.
Abstract
Culture medium or medium supplement is one of the factors responsible for dendritic cell (DC) generation, but little
is known about the influence of various media on DC culture. In our study we generated DC from adherent monocytes of
human peripheral blood in the presence of GM-CSF, IL-4 and TNF-α. The following culture media were used: RPMI 1640
supplemented with 2% human serum albumin; RPMI 1640 supplemented with 2% TCH serum replacement; X-VIVO 15 and
Panserin 501. Flow cytometry analysis revealed that in all media cells were CD83+ and lost CD14. Interestingly, the use of
Panserin and RPMI with albumin preferentially gave rise to CD1a+ DC, whereas in X-VIVO and RPMI with TCH we observed
both CD1a+ and CD1a-. Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both
CD80 and CD86 molecules and, in contrast to other media, the higher percentage of CD86+ cells in comparison to CD80+
cells.
Keywords: Dendritic cellsSerum-free mediumCell culture
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