open access

Vol 43, No 1 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-19
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Generation of dendritic cells from human peripheral blood monocytes - comparison of different culture media

Nataliya Tkachenko, Kamila Wojas, Jacek Tabarkiewicz, Jacek Rolinski
Folia Histochem Cytobiol 2005;43(1):25-30.

open access

Vol 43, No 1 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-19

Abstract

Culture medium or medium supplement is one of the factors responsible for dendritic cell (DC) generation, but little is known about the influence of various media on DC culture. In our study we generated DC from adherent monocytes of human peripheral blood in the presence of GM-CSF, IL-4 and TNF-α. The following culture media were used: RPMI 1640 supplemented with 2% human serum albumin; RPMI 1640 supplemented with 2% TCH serum replacement; X-VIVO 15 and Panserin 501. Flow cytometry analysis revealed that in all media cells were CD83+ and lost CD14. Interestingly, the use of Panserin and RPMI with albumin preferentially gave rise to CD1a+ DC, whereas in X-VIVO and RPMI with TCH we observed both CD1a+ and CD1a-. Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both CD80 and CD86 molecules and, in contrast to other media, the higher percentage of CD86+ cells in comparison to CD80+ cells.

Abstract

Culture medium or medium supplement is one of the factors responsible for dendritic cell (DC) generation, but little is known about the influence of various media on DC culture. In our study we generated DC from adherent monocytes of human peripheral blood in the presence of GM-CSF, IL-4 and TNF-α. The following culture media were used: RPMI 1640 supplemented with 2% human serum albumin; RPMI 1640 supplemented with 2% TCH serum replacement; X-VIVO 15 and Panserin 501. Flow cytometry analysis revealed that in all media cells were CD83+ and lost CD14. Interestingly, the use of Panserin and RPMI with albumin preferentially gave rise to CD1a+ DC, whereas in X-VIVO and RPMI with TCH we observed both CD1a+ and CD1a-. Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both CD80 and CD86 molecules and, in contrast to other media, the higher percentage of CD86+ cells in comparison to CD80+ cells.
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Keywords

Dendritic cells; Serum-free medium; Cell culture

About this article
Title

Generation of dendritic cells from human peripheral blood monocytes - comparison of different culture media

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 1 (2005)

Article type

Original paper

Pages

25-30

Published online

2011-08-19

Bibliographic record

Folia Histochem Cytobiol 2005;43(1):25-30.

Keywords

Dendritic cells
Serum-free medium
Cell culture

Authors

Nataliya Tkachenko
Kamila Wojas
Jacek Tabarkiewicz
Jacek Rolinski

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