Vol 43, No 2 (2005)
Original paper
Published online: 2011-08-22
Flow cytometric analysis of CD55 and CD59 expression on blood cells in paroxysmal nocturnal haemoglobinuria
Folia Histochem Cytobiol 2005;43(2):117-120.
Abstract
PNH is a rare clonal disorder of hematopoietic stem cells, therefore all blood cells lineages are involved. The main
feature is an increased sensitivity of erythrocytes to complement-mediated cell lysis due to deficiency of membrane-bound
GPI (glycosylphosphatidylinositol)-anchored proteins which normally function as inhibitors of reactive hemolysis. In the
present study, we performed flow cytometric analysis using monoclonal antibodies against CD55 and CD59 for the detection
of PNH-type clone in the blood of 50 patients (28 females and 22 males, age range 7-67 yrs). In one patient only we found a
large population (95%) of granulocytes with decreased expression of both CD55 and CD59 molecules (type I PNH) and in two
others with partial loss of CD55 expression (type II PNH). The expression was determined chiefly on granulocytes which in
the control group showed reliable and high expression of CD55 and CD59.
Keywords: Paroxysmal nocturnal haemoglobinuriaCD55CD59DAFMIRLHaemolytic anaemiaFlow cytometry
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