open access

Vol 43, No 2 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Flow cytometric analysis of CD55 and CD59 expression on blood cells in paroxysmal nocturnal haemoglobinuria

Grzegorz Dworacki, Jan Sikora, Ewa Mizera-Nyczak, Magdalena Trybus, Iwona Mozer-Lisewska, Anna Czyz, Jan Zeromski
Folia Histochem Cytobiol 2005;43(2):117-120.

open access

Vol 43, No 2 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

PNH is a rare clonal disorder of hematopoietic stem cells, therefore all blood cells lineages are involved. The main feature is an increased sensitivity of erythrocytes to complement-mediated cell lysis due to deficiency of membrane-bound GPI (glycosylphosphatidylinositol)-anchored proteins which normally function as inhibitors of reactive hemolysis. In the present study, we performed flow cytometric analysis using monoclonal antibodies against CD55 and CD59 for the detection of PNH-type clone in the blood of 50 patients (28 females and 22 males, age range 7-67 yrs). In one patient only we found a large population (95%) of granulocytes with decreased expression of both CD55 and CD59 molecules (type I PNH) and in two others with partial loss of CD55 expression (type II PNH). The expression was determined chiefly on granulocytes which in the control group showed reliable and high expression of CD55 and CD59.

Abstract

PNH is a rare clonal disorder of hematopoietic stem cells, therefore all blood cells lineages are involved. The main feature is an increased sensitivity of erythrocytes to complement-mediated cell lysis due to deficiency of membrane-bound GPI (glycosylphosphatidylinositol)-anchored proteins which normally function as inhibitors of reactive hemolysis. In the present study, we performed flow cytometric analysis using monoclonal antibodies against CD55 and CD59 for the detection of PNH-type clone in the blood of 50 patients (28 females and 22 males, age range 7-67 yrs). In one patient only we found a large population (95%) of granulocytes with decreased expression of both CD55 and CD59 molecules (type I PNH) and in two others with partial loss of CD55 expression (type II PNH). The expression was determined chiefly on granulocytes which in the control group showed reliable and high expression of CD55 and CD59.
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Keywords

Paroxysmal nocturnal haemoglobinuria; CD55; CD59; DAF; MIRL; Haemolytic anaemia; Flow cytometry

About this article
Title

Flow cytometric analysis of CD55 and CD59 expression on blood cells in paroxysmal nocturnal haemoglobinuria

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 2 (2005)

Article type

Original paper

Pages

117-120

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(2):117-120.

Keywords

Paroxysmal nocturnal haemoglobinuria
CD55
CD59
DAF
MIRL
Haemolytic anaemia
Flow cytometry

Authors

Grzegorz Dworacki
Jan Sikora
Ewa Mizera-Nyczak
Magdalena Trybus
Iwona Mozer-Lisewska
Anna Czyz
Jan Zeromski

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