open access

Vol 43, No 2 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Characterization of human pituitary adenomas in cell cultures by light and electron microscopic morphology and immunolabeling

Ilona Fazekas, Balázs Hegedűs, Ernő Bácsy, Edit Kerekes, Feliícia Slowik, Katalin Bálint, Emil Pásztor
Folia Histochem Cytobiol 2005;43(2):81-90.

open access

Vol 43, No 2 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

The morphology and hormone production of pituitary adenoma cell cultures were compared in order to highlight their characteristic in vitro features. Cell suspensions were prepared from 494 surgical specimens. The 319 viable monolayer cultures were analyzed in detail by light microscopy and immunocytochemistry within two weeks of cultivation. Some cultures were further characterized by scanning, transmission and immunogold electron microscopy. The viability and detailed in vitro morphology of adenoma cells were found to be characteristic for the various types of pituitary tumors. The sparsely granulated growth hormone, the corticotroph and the acidophil stem cell adenomas provided the highest ratio of viable cultures. Occasionally, prolonged maintenance of cells resulted in long-term cultures. Furthermore, a variety of particular distributions of different hormone-containing granules were found in several cases. Both light microscopic and ultrastructural analyses proved that the primary cultures of adenoma cells retain their physiological features during in vitro cultivations. Our in vitro findings correlated with the routine histopathological examination. These results prove that monolayer cultures of pituitary adenoma cells can contribute to the correct diagnosis and are valid model systems for various oncological and neuroendocrinological studies.

Abstract

The morphology and hormone production of pituitary adenoma cell cultures were compared in order to highlight their characteristic in vitro features. Cell suspensions were prepared from 494 surgical specimens. The 319 viable monolayer cultures were analyzed in detail by light microscopy and immunocytochemistry within two weeks of cultivation. Some cultures were further characterized by scanning, transmission and immunogold electron microscopy. The viability and detailed in vitro morphology of adenoma cells were found to be characteristic for the various types of pituitary tumors. The sparsely granulated growth hormone, the corticotroph and the acidophil stem cell adenomas provided the highest ratio of viable cultures. Occasionally, prolonged maintenance of cells resulted in long-term cultures. Furthermore, a variety of particular distributions of different hormone-containing granules were found in several cases. Both light microscopic and ultrastructural analyses proved that the primary cultures of adenoma cells retain their physiological features during in vitro cultivations. Our in vitro findings correlated with the routine histopathological examination. These results prove that monolayer cultures of pituitary adenoma cells can contribute to the correct diagnosis and are valid model systems for various oncological and neuroendocrinological studies.
Get Citation

Keywords

Pituitary adenoma; Hormone; Immunolabeling; Electron microscopy; Cell culture

About this article
Title

Characterization of human pituitary adenomas in cell cultures by light and electron microscopic morphology and immunolabeling

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 2 (2005)

Article type

Original paper

Pages

81-90

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(2):81-90.

Keywords

Pituitary adenoma
Hormone
Immunolabeling
Electron microscopy
Cell culture

Authors

Ilona Fazekas
Balázs Hegedűs
Ernő Bácsy
Edit Kerekes
Feliícia Slowik
Katalin Bálint
Emil Pásztor

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