open access

Vol 43, No 3 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Effect of aminoguanidine and albendazole on inducible nitric oxide synthase (iNOS) activity in T. spiralis-infected mice muscles

Jan Żeromski, Krystyna Boczoń, Elżbieta Wandurska-Nowak, Iwona Mozer-Lisewska
Folia Histochem Cytobiol 2005;43(3):157-159.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

The aim of this study was to provide evidence for the expression of iNOS in the cells of inflammatory infiltrates around larvae in skeletal muscles of T. spiralis infected mice. The BALB/c mice (n=8) divided into subgroups, received either aminoguanidine (AMG) - a specific iNOS inhibitor or albendazole (ALB) - an antiparasitic drug of choice in trichinellosis treatment. Control animals (n=2 in each subgroup) were either uninfected and treated or uninfected and untreated. Frozen sections of hind leg muscles from mice sacrificed at various time intervals after infection were cut and subjected to immunohistochemistry, using monoclonal anti-iNOS antibody. The ALB-treated mice revealed stronger iNOS staining in the infiltrating cells around larvae than the infected and untreated animals. On the contrary, in the AMG-treated animals, the infiltrating cells did not show any specific iNOS reaction. These data confirm the specificity of iNOS staining in the cellular infiltrates around T. spiralis larvae and shed some light on the role of nitric oxide during ALB treatment in experimental trichinellosis.

Abstract

The aim of this study was to provide evidence for the expression of iNOS in the cells of inflammatory infiltrates around larvae in skeletal muscles of T. spiralis infected mice. The BALB/c mice (n=8) divided into subgroups, received either aminoguanidine (AMG) - a specific iNOS inhibitor or albendazole (ALB) - an antiparasitic drug of choice in trichinellosis treatment. Control animals (n=2 in each subgroup) were either uninfected and treated or uninfected and untreated. Frozen sections of hind leg muscles from mice sacrificed at various time intervals after infection were cut and subjected to immunohistochemistry, using monoclonal anti-iNOS antibody. The ALB-treated mice revealed stronger iNOS staining in the infiltrating cells around larvae than the infected and untreated animals. On the contrary, in the AMG-treated animals, the infiltrating cells did not show any specific iNOS reaction. These data confirm the specificity of iNOS staining in the cellular infiltrates around T. spiralis larvae and shed some light on the role of nitric oxide during ALB treatment in experimental trichinellosis.
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Keywords

Aminoguanidine; Albendazole; iNOS; Trichnellosis; Muscle

About this article
Title

Effect of aminoguanidine and albendazole on inducible nitric oxide synthase (iNOS) activity in T. spiralis-infected mice muscles

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Article type

Original paper

Pages

157-159

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):157-159.

Keywords

Aminoguanidine
Albendazole
iNOS
Trichnellosis
Muscle

Authors

Jan Żeromski
Krystyna Boczoń
Elżbieta Wandurska-Nowak
Iwona Mozer-Lisewska

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