open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Published online: 2011-08-22
Submitted: 2011-12-19
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Effects of excess vitamin B6 intake on cerebral cortex neurons in rat: an ultrastructural study

Ramazan Demir, Goksemin Acar, Gamze Tanriover, Yasemin Seval, Umit Ali Kayisli, Aysel Agar
Folia Histochem Cytobiol 2005;43(3):143-150.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Published online: 2011-08-22
Submitted: 2011-12-19

Abstract

The aim of this study was to investigate whether excess of vitamin B6 leads to ultrastructural changes in cerebral cortex of forty-eight healthy albino rats which were included in the study. Saline solution was injected to to the control groups (CG-10, n=12 for 10 days; CG-15, n=12 for 15 days; CG-20, n=12 for 20 days). The three experimental groups (EG-10, n=12; EG-15, n=12; EG-20, n=12) were treated with 5 mg/kg vitamin B6 daily for 10 days (EG-10), 15 days (EG-15) and 20 days (EG-20). Brain tissues were prepared by glutaraldehyde-osmium tetroxide double fixation for ultrastructural analysis. No significant changes were observed in the control groups. The ultrastructural analysis revealed that the numbers of damaged mitochondria, lipofuscin granules and vacuoles were significantly higher in all the experimental groups than in the control groups (p<0.05). However, synaptic density was significantly decreased in the experimental groups as compared to the control groups (p<0.05). The results suggest that the excess of vitamin B6 intake causes damage to the cerebral cortex due to cellular intoxication and decreased synaptic density. Thus, careful attention should be paid to the time and dose of vitamin B6 recommended for patients who are supplemented with this vitamin

Abstract

The aim of this study was to investigate whether excess of vitamin B6 leads to ultrastructural changes in cerebral cortex of forty-eight healthy albino rats which were included in the study. Saline solution was injected to to the control groups (CG-10, n=12 for 10 days; CG-15, n=12 for 15 days; CG-20, n=12 for 20 days). The three experimental groups (EG-10, n=12; EG-15, n=12; EG-20, n=12) were treated with 5 mg/kg vitamin B6 daily for 10 days (EG-10), 15 days (EG-15) and 20 days (EG-20). Brain tissues were prepared by glutaraldehyde-osmium tetroxide double fixation for ultrastructural analysis. No significant changes were observed in the control groups. The ultrastructural analysis revealed that the numbers of damaged mitochondria, lipofuscin granules and vacuoles were significantly higher in all the experimental groups than in the control groups (p<0.05). However, synaptic density was significantly decreased in the experimental groups as compared to the control groups (p<0.05). The results suggest that the excess of vitamin B6 intake causes damage to the cerebral cortex due to cellular intoxication and decreased synaptic density. Thus, careful attention should be paid to the time and dose of vitamin B6 recommended for patients who are supplemented with this vitamin
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Keywords

Vitamin B6; Cerebral cortex; Neuron; Rat; Ultrastructure

About this article
Title

Effects of excess vitamin B6 intake on cerebral cortex neurons in rat: an ultrastructural study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Pages

143-150

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):143-150.

Keywords

Vitamin B6
Cerebral cortex
Neuron
Rat
Ultrastructure

Authors

Ramazan Demir
Goksemin Acar
Gamze Tanriover
Yasemin Seval
Umit Ali Kayisli
Aysel Agar

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